X-linkage is not a general inhibitor of tissue-specific gene expression in Drosophila melanogaster
X-linkage is not a general inhibitor of tissue-specific gene expression in Drosophila melanogaster
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DOI：
10.1038/hdy.2017.12
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发表时间：
2017-07-01
期刊：
影响因子：
3.8
通讯作者：
Parsch, J.
中科院分区：
文献类型：
--
作者：
Argyridou, E.;Huylmans, A. K.;Parsch, J.
由于男性和女性之间的拷贝数差异，X 染色体受到独特的进化力量和基因调控机制的影响。先前对果蝇的研究表明，X连锁、睾丸特异性报告基因的表达在雄性种系中受到抑制。然而，尚不清楚这种现象是否仅限于睾丸表达的基因，或者是否是具有组织特异性表达的基因的更普遍特性，这些基因在 X 染色体上的代表性也不足。为了测试这一点，我们比较了插入不同常染色体和 X 染色体位置的三种组织特异性报告基因（卵巢、副腺和马氏小管）的表达。与睾丸特异性报告基因相反，我们发现任何其他组织中的 X 连锁表达没有减少。在副腺和马氏小管中，我们检测到 X 连锁报告基因的表达较高，这表明它们至少部分是剂量补偿的。我们发现 X 连锁报告基因和常染色体报告基因的组织特异性没有差异。这些发现表明，一般来说，X 染色体对于组织特异性基因表达来说并不是一个有害的环境，并且 X 连锁表达的抑制仅限于雄性种系。
As a consequence of its difference in copy number between males and females, the X chromosome is subject to unique evolutionary forces and gene regulatory mechanisms. Previous studies of Drosophila melanogaster have shown that the expression of X-linked, testis-specific reporter genes is suppressed in the male germline. However, it is not known whether this phenomenon is restricted to testis-expressed genes or if it is a more general property of genes with tissue-specific expression, which are also underrepresented on the X chromosome. To test this, we compared the expression of three tissue-specific reporter genes (ovary, accessory gland and Malpighian tubule) inserted at various autosomal and X-chromosomal locations. In contrast to testis-specific reporter genes, we found no reduction of X-linked expression in any of the other tissues. In accessory gland and Malpighian tubule, we detected higher expression of the X-linked reporter genes, which suggests that they are at least partially dosage compensated. We found no difference in the tissue-specificity of X-linked and autosomal reporter genes. These findings indicate that, in general, the X chromosome is not a detrimental environment for tissue-specific gene expression and that the suppression of X-linked expression is limited to the male germline.