LncRNA SNHG12 promotes the proliferation and metastasis of papillary thyroid carcinoma cells through regulating wnt/β-catenin signaling pathway
LncRNA SNHG12 promotes the proliferation and metastasis of papillary thyroid carcinoma cells through regulating wnt/β-catenin signaling pathway
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DOI：
10.3233/cbm-170777
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发表时间：
2018-01-01
影响因子：
3.1
通讯作者：
Dang, Shuangsuo
中科院分区：
文献类型：
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作者：
Ding, Shimei;Qu, Wei;Dang, Shuangsuo
目的：探讨长非编码RNA(IncRNA)小核仁RNA宿主基因12(SNHG12)在甲状腺乳头状癌(PTC)中的表达及意义。方法：采用定量逆转录聚合酶链式反应(qRT-PCR)检测42对甲状腺乳头状癌(PTC)及癌旁组织中IncRNA SNHG12的相对表达水平。设计并合成了SNHG12特异性干扰序列。QRT-PCR检测SNHG12在PTC细胞中的相对表达水平和转染率。干预SNHG12表达后，用四甲基偶氮唑蓝(四甲基偶氮唑蓝)法检测细胞增殖能力的变化，用流式细胞仪检测细胞周期分布的变化，用Transwell法和创伤愈合实验检测细胞迁移和侵袭能力的变化，用Western blotting检测Wnt/βcatenin途径分子标志物的表达变化。建立裸鼠肺转移模型，通过体内实验研究SNHG12表达干扰后肿瘤细胞迁移和侵袭能力的变化。结果：qRT-PCR结果显示30对PTC组织和细胞中SNHG12表达上调。四甲基偶氮唑盐比色法结果显示，干扰SNHG12后，细胞增殖能力受到抑制。流式细胞仪检测结果显示，SNHG12表达下调后，细胞周期被阻滞在G1-G0期。Transwell实验和Western blotting结果表明，SNHG12干扰可通过影响Wnt/β-catenin信号通路而抑制肿瘤细胞的侵袭和转移能力。裸鼠转移瘤模型显示SNHG12可影响肿瘤细胞的体内侵袭和转移。结论：SNHG12在PTC组织和细胞中表达较高。体外实验证明SNHG12可通过影响Wnt/β-catenin信号通路促进PTC细胞的增殖和转移。
OBJECTIVE: To investigate the expression and role of long non-coding RNA (IncRNA) small nucleolar RNA host gene 12 (SNHG12) in papillary thyroid carcinoma (PTC).METHODS: The relative expression levels of IncRNA SNHG12 (hereinafter referred to as SNHG12) in 42 pairs of PTC tissues and para-carcinoma tissues were detected via quantitative reverse transcription polymerase chain reaction (qRT-PCR). SNHG12 specific interference sequences were designed and synthesized. The relative expression level and transfection efficiency of SNHG12 in PTC cells were detected via qRT-PCR. After the interference in SNHG12 expression, the change in cell proliferation capacity was detected via methyl thiazolyl tetrazolium (MTT) assay, the change in cell cycle distribution was detected via flow cytometry, the changes in cell migration and invasion capacities were detected via Transwell assay and wound healing assay, and the changes in expressions of molecular markers of Wnt/beta catenin pathway were detected via Western blotting. The pulmonary metastasis model of nude mice was established, and the changes in migration and invasion capacities of tumor cells were studied via the in-vivo experiment after the interference in SNHG12 expression.RESULTS: The results of qRT-PCR showed that the SNHG12 expression was up-regulated in 30 pairs of PTC tissues and cells. The results of MTT assay showed that the cell proliferation capacity was inhibited after the interference in SNHG12. The results of flow cytometry showed that the cell cycle progression was blocked in G1-G0 phase after the knockdown of SNHG12 expression. The results of Transwell assay and Western blotting showed that the interference in SNHG12 could inhibit the invasion and metastasis capacities of tumor cells through influencing the Wnt/beta-catenin signaling pathway. Metastatic tumor model of nude mice showed that SNHG12 could affect the invasion and metastasis of tumor cells in vivo.CONCLUSION: The SNHG12 expression is relatively high in PTC tissues and cells. In-vivolin-vitro experiments prove that SNHG12 can promote the proliferation and metastasis of PTC cells through influencing the Wnt/beta-catenin signaling pathway.