Pseudo-Ligandless Click Chemistry for Oligonucleotide Conjugation.
Pseudo-Ligandless Click Chemistry for Oligonucleotide Conjugation.
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用于寡核苷酸缀合的伪无配体点击化学。
DOI：
10.1002/cpch.1
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发表时间：
2016
影响因子：
--
通讯作者：
Das,SubhaR
中科院分区：
文献类型：
--
作者：
Mack,Stephanie;Fouz,MuniraF;Dey,SouravK;Das,SubhaR
Particularly for its use in bioconjugations, the copper‐catalyzed (or copper‐promoted) azide‐alkyne cycloaddition (CuAAC) reaction or ‘click chemistry’, has become an essential component of the modern chemical biologist's toolbox. Click chemistry has been applied to DNA, and more recently, RNA conjugations, and the protocols presented here can be used for either. The reaction can be carried out in aqueous buffer, and uses acetonitrile as a minor co‐solvent that serves as a ligand to stabilize the copper. The method also includes details on the analysis of the reaction product. © 2016 by John Wiley & Sons, Inc.
