A cost-practical cell-recycling process for xylonic acid bioproduction from acidic lignocellulosic hydrolysate with whole-cell catalysis of Gluconobacter oxydans
A cost-practical cell-recycling process for xylonic acid bioproduction from acidic lignocellulosic hydrolysate with whole-cell catalysis of Gluconobacter oxydans
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DOI：
10.1016/j.biortech.2021.125157
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发表时间：
2021-04-17
影响因子：
11.4
通讯作者：
Xu, Yong
中科院分区：
文献类型：
--
作者：
Han, Jian;Hua, Xia;Xu, Yong
木糖酸（XA）作为一种生物基平台化学品，在木糖生物转化中具有重要意义。葡萄糖酸氧化酶的全细胞催化具有很好的应用前景，但细胞培养的困难仍然严重阻碍了含毒素木质纤维素粗水解物的XA商业化。因此，细菌细胞应该被回收以降低商业生产成本。该方法不仅能吸收水解液中大部分降解的抑制剂，而且能将糖的损失控制在10%以内，使菌体在回收过程中保持90%的生物转化性能。此外，在密封供氧发酵罐中实现了XA生物生产的放大。最后，210克XA生产从1000克玉米芯来源的水解液在24小时内的全细胞催化。硅藻土处理为从木质纤维素生物质商业生物生产生物化学品如XA提供了有效且成本实用的方法。
Xylonic acid (XA), as a bio-based platform chemical, is of considerable interest for xylose bioconversion. The whole-cell catalysis of Gluconobacter oxydans presents a promising application potential, while the hard works of cell culture still severely hinder XA business from the crude toxics-containing lignocellulosic hydrolysate. Hence, the bacterial cells should be recycled to reduce commercial production cost. The implementation of diatomite detoxification not only absorbs most of the degraded inhibitors in hydrolysate, but also confines the sugar contents loss with 10% and allows the bacterial cells to maintain 90% bioconversion performance during cellrecycling operation. Additionally, a scale-up of XA bioproduction was achieved in a sealed oxygen supply fermenter. Finally, 210 g XA was produced from 1000 g corncob originated hydrolysate within 24 h of whole-cell catalysis. Diatomite treatment provides an efficient and cost-practical approach for the commercial bioproduction of biochemicals like XA from lignocellulosic biomass.