Reactivity with a specific epitope of outer surface protein A predicts protection from infection with the Lyme disease spirochete, Borrelia burgdorferi
Reactivity with a specific epitope of outer surface protein A predicts protection from infection with the Lyme disease spirochete, Borrelia burgdorferi
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DOI：
10.1128/iai.65.3.882-889.1997
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发表时间：
1997-03-01
影响因子：
3.1
通讯作者：
Frantz, JC
中科院分区：
文献类型：
--
作者：
Golde, WT;Piesman, J;Frantz, JC
研究了对莱姆病重组疫苗的应答，以确定有效保护免受蜱传播感染的血清抗体水平。本文提供的数据证明了针对外表面蛋白A（OspA）上的表位的抗体与犬和小鼠中针对伯氏疏螺旋体感染的保护之间的显著相关性。开发了竞争性酶联免疫吸附测定以测量针对OspA上的位点的抗体，所述位点由单克隆抗体LA-2定义，接种和攻击后，LA-2滴度对犬和小鼠感染的比较建立了LA-2滴度的预测值，用Logistic回归分析计算血清抗体水平与保护力之间的统计学关系。统计模型预测，LA-2滴度为0.32 μ g当量（eq）/ml，与小鼠和犬的80%预测保护概率相关。该值用于根据蜱暴露时的保护状态对小鼠和犬进行分类。LA-2临界滴度（0.32 μ g eq/ml）正确分类了所有未感染的犬（n = 13）和小鼠（n = 44）。相比之下，滴度低于0.32 μ g eq/ml的22只狗中有20只和31只小鼠中有28只被感染。基于这些结果，我们得出结论，LA-2滴度是一个可靠的免疫状态指标，用于估计使用基于OspA的疫苗后对B的免疫保护。严格意义上的伯格多夫
The response to recombinant vaccines for Lyme disease was studied to determine serum antibody levels effective in protecting against tick-transmitted infection. Data presented here demonstrate a significant correlation between antibody to an epitope on outer surface protein A (OspA) and protection against infection with Borrelia burgdorferi in canines and mice, A competitive enzyme-linked immunosorbent assay was developed to measure antibody to a site on OspA, defined by monoclonal antibody LA-2, Comparison of LA-2 titers against infection of canines and mice following vaccination and challenge established a predicted value for LA-2 titers, The statistical relationship between serum antibody levels and protection was calculated by logistic regression analysis. The statistical model predicted that an LA-2 titer of 0.32 mu g equivalents (eq) per mi correlated to an 80% predicted probability of protection for both mice and dogs. This value was used to classify mice and dogs as to their protected status at the time of tick exposure, The LA-2 cutoff titer (0.32 mu g eq/ml) correctly classified all dogs (n = 13) and mice (n = 44) that failed to become infected. By contrast, 20 of 22 dogs and 28 of 31 mice with titers of less than 0.32 mu g eq/ml became infected. On the basis of these results, we conclude that an LA-2 titer is a reliable indicator of immune status for estimating immune protection following use of OspA-based vaccines for B. burgdorferi sensu stricto.