γH2AX and its role in DNA double-strand break repair
γH2AX and its role in DNA double-strand break repair
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DOI：
10.1139/o06-072
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发表时间：
2006-08-01
影响因子：
2.9
通讯作者：
Krogan, Nevan J.
中科院分区：
文献类型：
--
作者：
Fillingham, Jeffrey;Keogh, Michael-Christopher;Krogan, Nevan J.
对DNA双链断裂（DSB）的最早反应之一是芽殖酵母H2 A（后生动物组蛋白H2 AX）的羧基末端磷酸化，以产生γ H2 A（或γ Eta 2 α X）。这种染色质修饰在DSB周围延伸了数十个千碱基，并且被认为在断裂识别和修复中发挥着多种作用，尽管它可能不是许多这些事件的主要信号。研究表明，γ H2 A（X）有两个更直接的作用：（i）在DSB周围招募粘附素，（ii）维持检查点阻滞。最近的工作已经确定了其他因素，包括染色质重塑和蛋白磷酸酶，其靶向γ H2 A（X）并调节DSB修复/恢复。
One of the earliest responses to a DNA double-strand break (DSB) is the carboxy-terminal phosphorylation of budding yeast H2A (metazoan histone H2AX) to create gamma H2A (or gamma Eta 2 alpha X). This chromatin modification stretches more than tens of kilobases around the DSB and has been proposed to play numerous roles in break recognition and repair, although it may not be the primary signal for many of these events. Studies suggest that gamma H2A(X) has 2 more direct roles: (i) to recruit cohesin around the DSB, and (ii) to maintain a checkpoint arrest. Recent work has identified other factors, including chromatin remodelers and protein phosphatases, which target gamma H2A(X) and regulate DSB repair/recovery.