Growth of osteoblasts on porous calcium phosphate ceramic: an in vitro model for biocompatibility study.
Growth of osteoblasts on porous calcium phosphate ceramic: an in vitro model for biocompatibility study.
复制标题
成骨细胞在多孔磷酸钙陶瓷上的生长：生物相容性研究的体外模型。
DOI：
10.1016/0142-9612(89)90012-4
复制
发表时间：
1989
期刊：
影响因子：
14
通讯作者：
Haak,MH
中科院分区：
文献类型：
--
作者：
Cheung,HS;Haak,MH
动物体内生物材料植入通常用于生物相容性研究以及组织与试验材料之间长期相互作用的检查。提出了一种动物体外细胞培养模型，它可以挽救动物的生命，减少动物的痛苦和不适。在这项研究中，生物材料与特定材料植入部位的典型细胞类型相匹配：多孔磷酸钙陶瓷，用作牙科和骨科植入物，具有骨膜成纤维细胞、成骨细胞和软骨细胞。所有三种细胞类型都附着在陶瓷上并形成多细胞层。骨膜成纤维细胞，成骨细胞和软骨细胞的数量增加了29-，23-和17倍，分别在10周期间。成骨细胞通过仅产生I型胶原蛋白来保持其表型表达。甲状旁腺激素（PTH，50 nM）抑制成骨细胞的碱性磷酸酶活性超过50%，并增加cAMP超过10倍的控制文化。
Biomaterial implantation in animals is commonly used for biocompatibility studies as well as examination of long-term interaction between tissue and the test material. Anin vitrocell culture model is proposed as an alternative which will save animal lives and reduce the pain and discomfort of animals used for such studies. In this study the biomaterial was matched to the cell types typical of the implant site of the particular material: porous calcium phosphate ceramic, used as dental and orthopaedic implants, with periosteal fibroblasts, osteoblasts and chondrocytes. All three cell types attached on to the ceramic and formed multicellular layers. Numbers of periosteal fibroblasts, osteoblasts and chondrocytes increased 29-, 23- and 17-fold, respectively, during the 10 wk period. Osteoblasts retained their phenotypic expression by producing only Type I collagen. Parathyroid hormone (PTH, 50 nM) suppressed the alkaline phosphatase activity of osteoblasts by over 50% and increased cAMP by more than 10-fold over control cultures.