Plant-mediated horizontal transmission of Rickettsia endosymbiont between different whitefly species
Plant-mediated horizontal transmission of Rickettsia endosymbiont between different whitefly species
复制标题
植物介导的立克次体内共生体在不同粉虱物种之间的水平传播
DOI：
10.1093/femsec/fix138
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发表时间：
2017-12-01
影响因子：
4.2
通讯作者：
Qiu, Bao-Li
中科院分区：
文献类型：
--
作者：
Li, Yi-Han;Ahmed, Muhammad Z.;Qiu, Bao-Li
越来越多的研究表明，在亲缘关系较远的节肢动物中存在密切相关的内共生菌，表明这些细菌的水平传播。在这里，我们调查了两个全球入侵烟粉虱种，烟粉虱MEAM 1和B之间的立克次体的种间水平传播。烟粉虱MED，通过棉花植物。我们在这些粉虱中发现了散在和局限的立克次体分布模式。立克次体进入棉花叶片后，仅限于叶片韧皮部导管，可被两种无立克次体粉虱成虫摄取，但在受体粉虱中仅观察到散在模式。MEAM1雌虫体内立克次体的相对数量和向棉叶传播立克次体的效率显著高于MED雌虫。利用16S rRNA和gltA基因进行系统发育分析，结果表明，从供体MEAM1、棉花叶片、受体MEAM1和受体MED中提取的立克次体均为同一种立克次体。我们的结论是，棉花植物可以介导不同昆虫物种之间的水平传播的立克次体，并传播动力学的立克次体不同的寄主粉虱物种。
A growing number of studies have revealed the presence of closely related endosymbionts in phylogenetically distant arthropods, indicating horizontal transmission of these bacteria. Here we investigated the interspecific horizontal transmission of Rickettsia between two globally invasive whitefly species, Bemisia tabaci MEAM1 and B. tabaci MED, via cotton plants. We found both scattered and confined distribution patterns of Rickettsia in these whiteflies. After entering cotton leaves, Rickettsia was restricted to the leaf phloem vessels and could be taken up by both species of the Rickettsia-free whitefly adults, but only the scattered pattern was observed in the recipient whiteflies. Both the relative quantity of Rickettsia and the efficiency of transmitting Rickettsia into cotton leaves were significantly higher in MEAM1 females than in MED females. The retention time of Rickettsia transmitted from MEAM1 into cotton leaves was at least 5 days longer than that of MED. Phylogenetic analysis based on 16S rRNA and gltA genes confirmed that the Rickettsia extracted from the donor MEAM1, the cotton leaves, the recipient MEAM1 and the recipient MED were all identical. We conclude that cotton plants can mediate horizontal transmission of Rickettsia between different insect species, and that the transmission dynamics of Rickettsia vary with different host whitefly species.