LIGHT AND ELECTRON-MICROSCOPIC IDENTIFICATION OF ELASTIC, ELAUNIN AND OXYTALAN FIBERS IN HUMAN TRACHEAL AND BRONCHIAL-MUCOSA
LIGHT AND ELECTRON-MICROSCOPIC IDENTIFICATION OF ELASTIC, ELAUNIN AND OXYTALAN FIBERS IN HUMAN TRACHEAL AND BRONCHIAL-MUCOSA
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DOI：
10.1007/bf00318999
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发表时间：
1984-01-01
期刊：
影响因子：
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通讯作者：
STOCKINGER, L
中科院分区：
文献类型：
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作者：
BOCK, P;STOCKINGER, L
用光镜和电子显微镜观察了人体气管和支气管粘膜中的弹性纤维系统。可分辨出弹性纤维、弹性纤维和氧化他兰纤维。这些纤维是通过它们的染色特征(弹性染色，二硫键方法)和它们的精细结构形态来鉴定的。弹性纤维由弹性蛋白和极少的“弹性纤维微纤维”组成。弹性纤维中弹性蛋白的相对含量(与弹性纤维微纤量相比)较多，而弹性纤维中弹性蛋白的相对含量较小。相比之下，Oxytalan纤维是一束纯净的微纤维。在光学显微镜下，一层清晰的弹性层将正常粘膜的固有层和粘膜下层隔开。弹力层是由纵向排列的粗股弹性纤维形成的。上皮层(上皮下弹力层)的基底膜上附着着一个由弹性纤维组成的微细网络。少数弹性蛋白阳性纤维发出分支，穿过增厚的基底膜区域，插入上皮的基底层。在固有层和粘膜下层均可见弹性蛋白阳性纤维的疏松网络。软骨板层、腺上皮层和平滑肌细胞束被纤细的弹力纤维包裹，电子显微镜下显示弹力纤维主要由弹性纤维组成，而弹性纤维来自上皮下弹力层。穿过增厚的上皮基底膜的纤维被鉴定为奥他兰纤维。所有三种类型的纤维都存在于孔板和粘膜下层。ELaunin纤维和Oxtalan纤维构成了围绕着腺上皮、平滑肌束和软骨的Elastica阳性网。Oxtalan纤维(在增厚的基底膜内)、Elaunin纤维(上皮下弹力纤维阳性层)和碎裂纤维(弹性板层)的首选位置有助于比较这些纤维的光镜染色反应和精细结构形态。
The elastic fiber system in the human tracheal and bronchial mucosa was studied by light and electron microscopy. Elastic fibers, elaunin fibers, and oxytalan fibers were discerned. These fibers were identified by means of their staining characteristics (elastica stains, methods for disulfide-groups) and on account of their fine structural morphology. Elastic fibers consist of elastin and few “elastic-fiber microfibrils”. The relative amount of elastin (compared to the amount of elastic-fiber microfibrils) is large in elastic fibers but small in elaunin fibers. Oxytalan fibers — by contrast — are pure bundles of microfibrils.In the light microscope a well-defined elastic lamina separates the lamina propria and the submucosa of the normal mucous membrane. The elastic lamina is formed by coarse strands of longitudinally running elastic fibers. A delicate network of elastica-positive fibers is attached to the basement membrane of the epithelial layer (subepithelial elastic layer). A few of these elastica-positive fibers branch out, traverse the region of the thickened basement membrane, and insert into the basal lamina of the epithelium. A loose network of elastica-positive fibers is present both in the lamina propria and in the submucosa. Plates of cartilage, glandular epithelium, and bundles of smooth muscle cells are enveloped by delicate elastica-positive fibers.Electron microscopy shows the lamina elastica to be predominantly composed of elastic fibers, whilst elaunin fibers from the subepithelial elastic layer. Fibers penetrating the thickened basement membrane of the epithelium are identified as oxytalan fibers. All three types of fibers are present throughout the lamina porpria and in the submucosa. Elaunin fibers and oxytalan fibers comprise the elastica-positive nets around glandular epithelium, smooth muscle bundles, and cartilage. The preferred location of oxytalan fibers (within the thickened basement membrane), elaunin fibers (subepithelial elastica-positive layer), and clastic fibers (lamina elastica) facilitates the comparison of light microscopic staining reactions and fine structural morphology of these fibers.