Biotin-conjugated N-methylisatoic anhydride: a chemical tool for nucleic acid separation by selective 2′ -hydroxyl acylation of RNA
Biotin-conjugated N-methylisatoic anhydride: a chemical tool for nucleic acid separation by selective 2′ -hydroxyl acylation of RNA
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DOI：
10.1039/c4cc01134a
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发表时间：
2014-01-01
影响因子：
4.9
通讯作者：
Laurent, A.
中科院分区：
文献类型：
--
作者：
Ursuegui, S.;Chivot, N.;Laurent, A.
与生物素和二硫键连接物缀合的靛红酸酐衍生物是专为核酸分离而设计的。从 DNA-RNA 混合物开始，对 RNA 进行选择性 2'-羟基酰化，然后用链霉亲和素包被的磁珠捕获并裂解二硫键，从而洗脱 RNA。
An isatoic anhydride derivative conjugated to a biotin and a disulfide linker was specifically designed for the separation of nucleic acids. Starting from a DNA-RNA mixture, a selective 2 '-hydroxyl acylation of RNAs followed by capture with streptavidin-coated magnetic beads and cleavage of the disulfide led to elution of RNAs.