Raman spectroscopy and support vector machines for quick toxicological evaluation of titania nanoparticles
Raman spectroscopy and support vector machines for quick toxicological evaluation of titania nanoparticles
复制标题
拉曼光谱和支持向量机用于二氧化钛纳米颗粒的快速毒理学评估
DOI：
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
B. Moudgil
中科院分区：
文献类型：
--
作者：
G. Pyrgiotakis;O. Kundakcioglu;P. Pardalos;B. Moudgil
随着纳米技术产品的迅速发展，纳米技术可能带来的不良影响引起了人们的极大关注。传统的生物试验通常用于评估体外毒性。然而，关于这些测定法的适用性存在问题，主要是由于所利用的染料与颗粒的潜在相互作用。此外，由于必须使用大量不同的测定方法，该过程可能昂贵且耗时。为了解决其中的一些问题，本研究使用拉曼光谱来研究粒子-细胞的相互作用。活细胞的光谱是与其化学成分直接相关的非常复杂和丰富的数据集合。为了提高数据分辨率并使毒性检测更加稳健，数据挖掘技术已经被部署。此外，数据挖掘技术实现了整个过程的完全自动化，最大限度地减少了用户输入。拉曼光谱通过逐峰分析和支持向量机的实现成功地评估了TiO2纳米颗粒的毒性。暴露36小时后，发现这些颗粒显示出细胞毒性。结果经MTT（3-（4,5-二甲基噻唑-2-酰基）-2,5-二苯四唑溴）测定证实，与现有文献一致。总的来说，拉曼光谱似乎是少数几个表现出低水平干扰（遮挡、荧光、发射等）的技术之一。由于它不依赖于生物标志物，它可以在原位使用较长时间，对细胞生物化学的影响最小。版权所有©2011 John Wiley & Sons， Ltd
With the rapid development of nanotechnology products, there is a significant concern on the adverse effects that might be associated with them. Traditional biological assays are typically used to asses the toxicity in vitro. There are, however, questions regarding the suitability of these assays for this purpose, mainly due to the potential interaction of the particles with the utilized dyes. In addition, this process can be costly and time consuming, as a large number of different assays have to be used. To address some of these issues, Raman spectroscopy is used in this study to investigate the particle-cell interactions. The spectrum of a living cell is a very complex and rich collection of data directly related to its chemical composition. To enhance the data resolution and make the detection of toxicity more robust, data-mining techniques have been deployed. Furthermore, data-mining techniques enable full automation of the entire process, minimizing user input. The Raman spectroscopy successfully evaluated the toxicity of TiO2 nanoparticles by both the peak-by-peak analysis and with the implementation of support vector machines. The particles were found to display cytotoxicity after 36 h of exposure. The results were confirmed by MTT (3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyltetrazolium Bromide) assay and are in agreement with the existing literature on the subject. Overall, Raman spectroscopy appears to be among the very few techniques that exhibit low levels of interferences (obscuration, fluorescence, emission, etc.) from the particle addition. Since it does not rely on biomarkers, it can be used in situ for an extended period with minimal effects on the cellular biochemistry. Copyright © 2011 John Wiley & Sons, Ltd.
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DOI：
10.1073/pnas.97.1.262
发表时间：
2000-01-04
影响因子：
11.1
作者：
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DOI：
10.1165/ajrcmb.24.2.4081
发表时间：
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