Isolation and culture of microvascular endothelial cells from murine spinal cord
Isolation and culture of microvascular endothelial cells from murine spinal cord
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DOI：
10.1016/j.jneuroim.2006.05.012
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发表时间：
2006-08-01
影响因子：
3.3
通讯作者：
Pachter, Joel S.
中科院分区：
文献类型：
--
作者：
Ge, Shujun;Pachter, Joel S.
本文报道了脊髓微血管内皮细胞（SCMEC）的分离和培养。尽管在形态上与形成血脑屏障（BBB）的脑微血管内皮细胞（BMEC）相似，但SCMEC表达的几种主要BBB蛋白的量减少，包括紧密连接相关蛋白ZO-1和闭合蛋白、粘附连接相关蛋白β-连环蛋白和VE-钙粘蛋白以及外排转运蛋白P-糖蛋白。这些区别特征可能反映了BBB和BSCB之间影响生理和病理生理过程的更广泛的差异。(c)2006 Elsevier B.V保留所有权利。
The isolation and culture of spinal cord microvascular endothelial cells (SCMEC), which form the blood-spinal cord barrier (BSCB), is described. Though morphologically similar to brain microvacular endothelial cells (BMEC) that form the blood-brain barrier (BBB), SCMEC express reduced amounts of several prominent BBB proteins, including tight junction-associated proteins ZO-1 and occludin, adherens junction-associated proteins beta-catenin and VE-cadherin, and the efflux transporter P-glycoprotein. These distinguishing features may reflect more widespread differences between the BBB and BSCB that impact physiological and pathophysiological processes. (c) 2006 Elsevier B.V All rights reserved.