Substance P conjugated to CdTe quantum dots triggers cytosolic calcium concentration oscillations and induces quantum dots internalization in the pancreatic carcinoma cell line AR4-2J
Substance P conjugated to CdTe quantum dots triggers cytosolic calcium concentration oscillations and induces quantum dots internalization in the pancreatic carcinoma cell line AR4-2J
复制标题
与 CdTe 量子点缀合的物质 P 触发胰腺癌细胞系 AR4-2J 中的胞质钙浓度振荡并诱导量子点内化
DOI：
10.1007/s00216-011-4980-9
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发表时间：
2011-07-01
影响因子：
4.3
通讯作者：
Cui, Zong Jie
中科院分区：
文献类型：
--
作者：
Duan, Yu Jiao;Liang, Hui Yuan;Cui, Zong Jie
用水溶液法制备了3-巯基丙酸稳定的高荧光量子点，并将其作为P物质(SP)的荧光标记，研究了SP与表达于AR4-2J细胞上的NK-1受体的相互作用。观察到CDTe量子点在AR4-2J细胞膜上的非特异性吸附，而QD-SP偶联物成功地穿过细胞膜进入胞浆。SP是一种神经递质，神经递质诱导的钙浓度振荡是各种细胞尤其是分泌型细胞中普遍存在的现象。本文研究了SP和QD-SP结合物刺激AR4-2J细胞时细胞内钙离子浓度的变化。SP和QD-SP偶联物引发的振荡具有剂量依赖性，且非常相似。这种QD-SP结合物很容易内化到胞浆中，就像活性的NK-1配体所预期的那样。因此，QD-SP结合物可以成功地用于研究活细胞中配体和NK-1受体的相互作用。我们的研究可能为同类研究提供有意义的参考。图：(A)3-Mpa封端的CdTe量子点或(B)QD-SP共轭化合物后AR4-2J细胞的共聚焦显微镜图像。(A)3-Mpa修饰的CdTe量子点没有引起AR4-2J细胞胞浆内钙浓度的任何增加，而(B)QD-SP结合物引发AR4-2J细胞内钙浓度的振荡增加，且呈剂量依赖关系
Highly fluorescent CdTe quantum dots (QDs) stabilized by 3-mercaptopropionic acid were prepared by an aqueous solution approach and used as a fluorescent label to link substance P (SP) in studying the interaction of SP with NK-1 receptor, which was expressed on the AR4-2J cell line. Nonspecific adsorptions of CdTe QDs on the AR4-2J cell membrane were observed, whereas the QD–SP conjugates successfully crossed the cell membrane and entered the cytosol. SP is a neurotransmitter, and neurotransmitter-induced calcium concentration oscillation is a common phenomenon in diverse cells especially of secretory type. Cytosolic calcium concentration responses were studied in the AR4-2J cell line during stimulation with SP and QD–SP conjugates. The oscillations triggered by SP and QD–SP conjugates were dose-dependent and very similar. Such QD–SP conjugates readily internalized into the cytosol as would be expected of an active NK-1 ligand. Therefore QD–SP conjugates could be used successfully to study ligand and NK-1 receptor interactions in live cells. Our research may provide a meaningful reference for congener research.FigureConfocal microscopic images of AR4-2J cells after addition of (A) 3-MPA-capped CdTe QDs or (B) QD-SP conjugates. (A) 3-MPA-capped CdTe QDs did not induce any increases in cytosolic calcium concentration in AR4-2J cells, while (B) QD-SP conjugates triggered oscillatory intracellular calcium increases dose-dependent in AR4-2J cells