Lipidomics characterization of the mechanism of Cynomorium songaricum polysaccharide on treating type 2 diabetes
Lipidomics characterization of the mechanism of Cynomorium songaricum polysaccharide on treating type 2 diabetes
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DOI：
10.1016/j.jchromb.2021.122737
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发表时间：
2021-05-28
影响因子：
3
通讯作者：
Chen, Lin
中科院分区：
文献类型：
--
作者：
Shi, Zi-Qi;Wang, Lu-Yi;Chen, Lin
虽然Cynomorium songaricum Rupr.多糖（CSP）对血糖调节的作用已被研究，但其潜在机制仍不清楚。为了解决这个问题，开发了基于MS的脂质组学策略，以获得CSP改善2型糖尿病（T2 DM）的机制的系统水平的理解。使用UPLC-QTOF/MS和多变量统计工具来鉴定与T2 DM相关的血清代谢物的变化以及对CSP治疗的反应。结果，在血清中发现并鉴定了35种潜在的生物标志物，其中26种代谢物在给予CSP后被调节到正常水平。通过对代谢途径的分析，认为甘油磷脂代谢与其代谢密切相关。这些结果表明，CSP的摄入表现出良好的抗糖尿病活性，主要是由于调节磷脂代谢，包括磷脂酰胆碱，溶血磷脂酰胆碱，磷脂酰乙醇胺和鞘磷脂。
Although Cynomorium songaricum Rupr. polysaccharide (CSP) has been examined for its effects on glucose regulation, its underlying mechanism is still unclear. To address this issue, a MS-based lipidomics strategy was developed to gain a system-level understanding of the mechanism of CSP on improving type 2 diabetes mellitus (T2DM). UPLC-QTOF/MS and multivariate statistical tools were used to identify the alteration of serum metabolites associated with T2DM and responses to CSP treatment. As a result, 35 potential biomarkers were found and identified in serum, amongst which 26 metabolites were regulated to normal like levels after the administration of CSP. By analyzing the metabolic pathways, glycerophospholipid metabolism was suggested to be closely involved. These results indicated that the intake of CSP exhibited promising anti-diabetic activity, largely due to the regulation of phospholipid metabolism, including phosphatidylcholines, lysophosphatydylcholines, phosphtatidylethanolamines and sphingomyelins.