Activation of Ralfuranone/Ralstonin Production by Plant Sugars Functions in the Virulence of Ralstonia solanacearum
Activation of Ralfuranone/Ralstonin Production by Plant Sugars Functions in the Virulence of Ralstonia solanacearum
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DOI：
10.1021/acschembio.9b00301
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发表时间：
2019-07-01
影响因子：
4
通讯作者：
Kai, Kenji
中科院分区：
文献类型：
--
作者：
Ishikawa, Yoko;Murai, Yuta;Kai, Kenji
植物病原细菌具有复杂的机制，通过感应寄主衍生的化合物来检测寄主植物的存在。青枯雷尔氏菌（Ralstonia solanacearum）是茄科植物上的细菌性枯萎病的病原菌，其利用群体感应来控制次生代谢产物ralfuranones/ralstonins的产生，这被认为与毒力有关。在这里，我们报告，D-半乳糖和D-葡萄糖，植物糖，激活生产的ralfuranones/ralstonins在R青枯菌。结果发现两个新的衍生物：Ralfuranone M（1）和ralstonin C（2），并通过光谱和化学方法鉴定了它们的结构。Ralstonin C（2）是一种环状脂肽，含有一个独特的脂肪酸（2S，3S，Z）-3-氨基-2-羟基二十碳-13-烯酸，而Ralfuranone M（1）与其他Ralfuranone具有共同的芳基-呋喃酮结构。D-半乳糖和D-葡萄糖激活了生物合成的ralfuranone/ralstonin基因的表达，并部分成为ralfuranones/ralstonins的生物合成来源。从受感染的番茄植株的木质部流体中检测到Ralfuranones和ralstonins，并且其产生缺陷的突变体对番茄和烟草植株表现出降低的毒性。综上所述，这些结果表明，宿主糖激活的ralfuranone/ralstonin生产功能在R。青枯菌毒力
Plant pathogenic bacteria possess sophisticated mechanisms to detect the presence of host plants by sensing host-derived compounds. Ralstonia solanacearum, the causative agent of bacterial wilt on solanaceous plants, employs quorum sensing to control the production of the secondary metabolite ralfuranones/ralstonins, which have been suggested to be involved in virulence. Here, we report that D-galactose and D-glucose, plant sugars, activate the production of ralfuranones/ralstonins in R solanacearum. As a result, two new derivatives, ralfuranone M (1) and ralstonin C (2), were found in the culture extracts, and their structures were elucidated by spectroscopic and chemical methods. Ralstonin C (2) is a cyclic lipopeptide containing a unique fatty acid, (2S,3S,Z)-3-amino-2-hydroxyicos-13-enoic acid, whereas ralfuranone M (1) has a common aryl-furanone structure with other ralfuranones. D-Galactose and D-glucose activated the expression of the biosynthetic ralfuranone/ralstonin genes and in part became the biosynthetic source of ralfuranones/ralstonins. Ralfuranones and ralstonins were detected from the xylem fluid of the infected tomato plants, and their production-deficient mutants exhibited reduced virulence on tomato and tobacco plants. Taken together, these results suggest that activation of ralfuranone/ralstonin production by host sugars functions in R. solanacearum virulence.