Preclinical Toxicology Studies of Recombinant Human Platelet-Derived Growth Factor-BB Either Alone or in Combination with Beta-Tricalcium Phosphate and Type I Collagen.
Preclinical Toxicology Studies of Recombinant Human Platelet-Derived Growth Factor-BB Either Alone or in Combination with Beta-Tricalcium Phosphate and Type I Collagen.
复制标题
重组人血小板衍生的生长因子BB的临床前毒理学研究或与β-三磷酸β-磷酸β-型胶原蛋白结合使用。
DOI：
10.4061/2010/246215
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发表时间：
2011-01-10
影响因子：
8.2
通讯作者：
Hollinger JO
中科院分区：
文献类型：
--
作者：
Young CS;Bradica G;Hart CE;Karunanidhi A;Street RM;Schutte L;Hollinger JO
人血小板衍生生长因子-BB（hPDGF-BB）是一种在损伤部位从血小板释放的碱性多肽生长因子。它是一种多功能分子，调节DNA合成和细胞分裂，并诱导与组织修复、动脉粥样硬化、炎症反应和肿瘤疾病有关的生物学效应。本文概述了从广泛的测试平台产生的毒理学数据，以确定rhPDGF-BB给药后的骨、软组织和全身反应。此外，研究了重组人PDGF-BB（rhPDGF-BB）与β-磷酸三钙（β-TCP）或胶原蛋白与β-TCP联合给药的全身和局部毒性，以确定皮肤致敏性、刺激性、肌内组织反应、致热原性、遗传毒性和溶血性。所有数据都强烈表明，rhPDGF-BB单独或与β-TCP或胶原与β-TCP组合是生物相容的，并且既没有全身毒性也没有局部毒性，支持其在促进患者伤口愈合中的安全使用。
Human platelet-derived growth factor-BB (hPDGF-BB) is a basic polypeptide growth factor released from platelets at the injury site. It is a multifunctional molecule that regulates DNA synthesis and cell division and induces biological effects that are implicated in tissue repair, atherosclerosis, inflammatory responses, and neoplastic diseases. This paper is an overview of the toxicology data generated from a broad testing platform to determine bone, soft tissue, and systemic responses following administration of rhPDGF-BB. Moreover, the systemic and local toxicity of recombinant human PDGF-BB (rhPDGF-BB) in combination with either beta-tricalcium phosphate (β-TCP) or collagen combined with β-TCP was studied to determine dermal sensitization, irritation, intramuscular tissue responses, pyrogenicity, genotoxicity, and hemolytic properties. All data strongly suggest that rhPDGF-BB either alone or in combination with β-TCP or collagen with β-TCP is biocompatible and has neither systemic nor local toxicity, supporting its safe use in enhancing wound healing in patients.