Six distinct nuclear factors interact with the 75-base-pair repeat of the Moloney murine leukemia virus enhancer.
Six distinct nuclear factors interact with the 75-base-pair repeat of the Moloney murine leukemia virus enhancer.
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六个不同的核因子与莫洛尼鼠白血病病毒增强子的 75 个碱基对重复相互作用。
DOI：
10.1128/mcb.7.3.1101
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发表时间：
1987
影响因子：
5.3
通讯作者：
D. Baltimore
中科院分区：
文献类型：
--
作者：
N. Speck;D. Baltimore
莫洛尼鼠白血病病毒增强子的 75 个碱基对重复序列上的 6 个不同核因子的结合位点已通过电泳迁移率变动分析与甲基化干扰相结合进行了鉴定。在 WEHI 231 核提取物中发现的其中三个因子，我们将其命名为 LVa、LVb 和 LVc（白血病病毒因子 a、b 和 c），之前尚未被识别。还检测到与保守的猿病毒 40 corelike 基序、NF-1 基序和糖皮质激素反应元件结合的核因子。对多个细胞系的测试表明，大多数因子似乎普遍存在，除了在 MEL 细胞的核提取物中以及在胚胎癌细胞系 PCC4 和 F9 中均未发现 NF-1 结合因子，而且 MEL 和 F9 核提取物中核心结合因子相对较少。
Binding sites for six distinct nuclear factors on the 75-base-pair repeat of the Moloney murine leukemia virus enhancer have been identified by an electrophoretic mobility shift assay combined with methylation interference. Three of these factors, found in WEHI 231 nuclear extracts, which we have named LVa, LVb, and LVc (for leukemia virus factors a, b, and c) have not been previously identified. Nuclear factors that bind to the conserved simian virus 40 corelike motif, the NF-1 motif, and the glucocorticoid response element were also detected. Testing of multiple cell lines showed that most factors appeared ubiquitous, except that the NF-1 binding factor was found neither in nuclear extracts from MEL cells nor in the embryonal carcinoma cell lines PCC4 and F9, and core-binding factor was relatively depleted from MEL and F9 nuclear extracts.
DOI：
10.1073/pnas.81.9.2650
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
PARSLOW, TG;BLAIR, DL;GRANNER, DK
通讯作者：
GRANNER, DK
有效感染 F9 胚胎癌细胞的多瘤突变体不能拯救 F9 细胞中的野生型多瘤。
DOI：
10.1073/pnas.79.5.1479
发表时间：
1982
影响因子：
11.1
作者：
Fujimura,FK;Linney,E
通讯作者：
Linney,E