Developmental toxicity of PCB 126 (3,3',4,4',5-pentachlorobiphenyl) in nestling American kestrels (Falco sparverius).
Developmental toxicity of PCB 126 (3,3',4,4',5-pentachlorobiphenyl) in nestling American kestrels (Falco sparverius).
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DOI：
10.1093/toxsci/34.2.188
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发表时间：
1996-12
期刊：
影响因子：
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通讯作者：
D. Hoffman;M. Melancon;P. Klein;C. Rice;J. Eisemann;R. K. Hines;J. Spann;G. Pendleton
中科院分区：
文献类型：
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作者：
D. Hoffman;M. Melancon;P. Klein;C. Rice;J. Eisemann;R. K. Hines;J. Spann;G. Pendleton
平面多氯联苯同系物对包括美洲红隼在内的鸟类具有胚胎毒性和致畸作用。以雏鹰为模型，研究了3，3‘，4，4’，5-五氯联苯(PCB126)的发育毒性。给雏鸟口服玉米油5微l/g体重(对照组)或平面PCB126，浓度为50,250或1000 ng/g体重，连续10天。剂量为50 ng/g的多氯联苯126导致肝脏浓度为156 ng/g湿重，肝脏肿大和轻度凝固性坏死，肝微粒体乙氧基二硫醚-O-脱烷基酶和苯氧基二苯基二硫醚-O-脱烷基酶增加10倍以上，甲氧基二硫醚-O-脱烷基酶增加约5倍。在该剂量下，脾的轻度至中度淋巴组织耗竭明显，甲状腺滤泡大小和含量减少。250 ng/g时，肝组织中PCB126浓度为380 ng/g，表现为多灶性凝固性坏死增加，骨生长减少，脾重量减轻，脾和法氏囊淋巴细胞耗竭，甲状腺退行性病变。在1000 ng/g时，肝脏浓度为1098 ng/g，伴随着法氏囊重量的减少，肝脏硫醇浓度的降低，以及血浆中ALT、AST和乳酸脱氢酶-L活性的升高。肝脏多氯联苯126浓度与氧化/还原谷胱甘肽的比率之间存在高度显著的正相关。这些发现表明，雏鸟比成鸟更容易受到多氯联苯126的毒害，但对胚胎的敏感度较低，平面多氯联苯对雏鸟有潜在的危险。
Planar PCB congeners are embryotoxic and teratogenic to birds including American kestrels. The developmental toxicity of 3,3',4,4',5-pentachlorobiphenyl (PCB 126) was studied in the posthatching kestrel as a model for the eagle. Nestlings were dosed orally for 10 days with 5 microl/g body weight of corn oil (controls) or the planar PCB 126 at concentrations of 50, 250, or 1000 ng/g body weight. Dosing with 50 ng/g of PCB 126 resulted in a hepatic concentration of 156 ng/g wet weight, liver enlargement and mild coagulative necrosis, over 10-fold increases in hepatic microsomal ethoxyresorufin-O-dealkylase and benzyloxyresorufin-O-dealkylase, and approximately a 5-fold increase in methoxyresorufin-O-dealkylase. At this dose, mild to moderate lymphoid depletion of the spleen was apparent, as were decreased follicle size and content of the thyroid. At 250 ng/g, concentration of PCB 126 in the liver was 380 ng/g with increasing multifocal coagulative necrosis, decreased bone growth, decreased spleen weight with lymphocyte depletion of the spleen and bursa, and degenerative lesions of the thyroid. At 1000 ng/g, the liver concentration was 1098 ng/g, accompanied by decreased bursa weight, decreased hepatic thiol concentration, and increased plasma enzyme activities (ALT, AST, and LDH-L) in addition to the previous effects. Highly significant positive correlations were noted between liver concentrations of PCB 126 and the ratio of oxidized to reduced glutathone. These findings indicate that nestling kestrels are more susceptible to PCB 126 toxicity than adults, but less sensitive than embryos, and that planar PCBs are of potential hazard to nestling birds.