Interaction between SWP9 and Polar Tube Proteins of the Microsporidian Nosema bombycis and Function of SWP9 as a Scaffolding Protein Contribute to Polar Tube Tethering to the Spore Wall
Interaction between SWP9 and Polar Tube Proteins of the Microsporidian Nosema bombycis and Function of SWP9 as a Scaffolding Protein Contribute to Polar Tube Tethering to the Spore Wall
复制标题
SWP9 和家蚕微孢子虫极管蛋白之间的相互作用以及 SWP9 作为支架蛋白的功能有助于极管束缚到孢子壁
DOI：
10.1128/iai.00872-16
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发表时间：
2016-12
影响因子：
3.1
通讯作者：
Pan Guoqing
中科院分区：
文献类型：
--
作者：
Yang Donglin;Pan Lixia;Peng Pai;Dang Xiaoqun;Li Chunfeng;Li Tian;Long Mengxian;Chen Jie;Wu Yujiao;Du Huihui;Luo Bo;Song Yue;Tian Rui;Luo Jie;Zhou Zeyang;Pan Guoqing
摘要微孢子虫具有独特的、高度特化的侵入机制，包括极管和孢子壁。孢子壁蛋白（SWPs）和极管蛋白（PTPs）在极管和孢子壁的形成、排列、有序定位和功能中的相互作用仍有待确定。本研究旨在研究家蚕微孢子虫（Nosema bombycis）SWP 7（NbSWP 7）、NbSWP 9和PTPs之间的蛋白质相互作用。免疫共沉淀，液相色谱-串联质谱（LC-MS/MS），和酵母双杂交数据表明，NbSWP 9，但不是NbSWP 7，与NbPTP 1和NbPTP 2相互作用。免疫电镜（IEM）结果显示NbSWP 9主要定位于成孢子细胞发育中的极管中，而NbSWP 7则随机定位于细胞质中。而NbSWP 9和NbSWP 7均定位于N.家蚕成熟孢子。NbSWP 7定位于极管的原因可能是由于NbSWP 9和NbSWP 7之间的相互作用。有趣的是，当成熟孢子在弱碱性环境刺激下萌发时，NbSWP 9而不是NbSWP 7的大部分积累在被挤出的极管的开始部分和破裂的孢子壁（称为锚定盘（AD））中。另外，抗NbSWP 9抗体以剂量依赖性方式减少孢子萌发。总之，我们的研究进一步证实了NbSWP 9是一种支架蛋白，不仅锚定和保持极管，而且还将极管拴在孢子壁上。
ABSTRACT All microsporidia possess a unique, highly specialized invasion mechanism that involves the polar tube and spore wall. The interaction between spore wall proteins (SWPs) and polar tube proteins (PTPs) in the formation, arrangement, orderly orientation, and function of the polar tube and spore wall remains to be determined. This study was undertaken to examine the protein interactions of Nosema bombycis SWP7 (NbSWP7), NbSWP9, and PTPs. Coimmunoprecipitation, liquid chromatography-tandem mass spectrometry (LC-MS/MS), and yeast two-hybrid data demonstrated that NbSWP9, but not NbSWP7, interacts with NbPTP1 and NbPTP2. Furthermore, immunoelectron microscopy (IEM) showed that NbSWP9 was localized mainly in the developing polar tube of sporoblasts, while NbSWP7 was found randomly in the cytoplasm. However, both NbSWP9 and NbSWP7 were located in the polar tube and spore wall of N. bombycis mature spores. The reason why NbSWP7 was localized to the polar tube may be due to the interaction between NbSWP9 and NbSWP7. Interestingly, the majority of NbSWP9, but not NbSWP7, accumulated in the beginning part of the extruded polar tube and the ruptured spore wall called the anchoring disk (AD) when the mature spores germinated under weak-alkaline environmental stimulation. Additionally, anti-NbSWP9 antibody reduced spore germination in a dose-dependent manner. In conclusion, our study further confirmed that NbSWP9 is a scaffolding protein that not only anchors and holds the polar tube but also tethers the polar tube to the spore wall.
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影响因子：
4
作者：
Peuvel-Fanget, I;Polonais, V;Delbac, F
通讯作者：
Delbac, F
影响因子：
2
作者：
V. Taupin;G. Méténier;C. Vivarès;G. Prensier
通讯作者：
V. Taupin;G. Méténier;C. Vivarès;G. Prensier
影响因子：
--
作者：
Southern, Timothy R.;Jolly, Carrie E.;Hayman, J. Russell
通讯作者：
Hayman, J. Russell
影响因子：
3.1
作者：
Donglin Yang;G. Pan;Xiaoqun Dang;Yawei Shi;Chunfeng Li;Pai Peng;Bo Luo;Maofei Bian;Yue Song-Yue
通讯作者：
Donglin Yang;G. Pan;Xiaoqun Dang;Yawei Shi;Chunfeng Li;Pai Peng;Bo Luo;Maofei Bian;Yue Song-Yue
影响因子：
3.1
作者：
Bouzahzah, Boumediene;Nagajyothi, Fnu;Weiss, Louis M.
通讯作者：
Weiss, Louis M.