Excitotoxic injury induces production of monocyte chemoattractant protein-1 in rat cortico-striatal slice cultures
Excitotoxic injury induces production of monocyte chemoattractant protein-1 in rat cortico-striatal slice cultures
复制标题
兴奋性毒性损伤诱导大鼠皮质纹状体切片培养物中单核细胞趋化蛋白-1 的产生
DOI：
10.1016/s0304-3940(02)00550-5
复制
发表时间：
2002
影响因子：
2.5
通讯作者：
M. Satoh
中科院分区：
文献类型：
--
作者：
Takahiro Katayama;M. Minami;Mika Nakamura;Misato Ito;H. Katsuki;A. Akaike;M. Satoh
在大鼠皮质-纹状体切片培养中研究了兴奋性毒性损伤对单核细胞趋化蛋白-1（MCP-1）产生的影响。用50 μM N-甲基-d-天冬氨酸（NMDA）处理4 h，引起神经元严重损伤，诱导星形胶质细胞产生MCP-1。处理后，生产水平立即显著升高，在4-8小时达到峰值，并在72小时降至接近基础水平。由于处理促进了切片培养物中MCP-1的释放，但在富集的星形胶质细胞培养物中没有，因此NMDA不太可能直接作用于星形胶质细胞。这些结果表明，NMDA诱导的神经元损伤的信息传递到星形胶质细胞，诱导MCP-1的产生。器官型切片培养可用于研究星形胶质细胞在神经元损伤过程中的炎症反应。
The effect of excitotoxic injury on the production of monocyte chemoattractant protein-1 (MCP-1) was examined in rat cortico-striatal slice cultures. Treatment with 50 μM N-methyl-d-aspartate (NMDA) for 4 h, which caused severe damage in neurons, induced the production of MCP-1 in astrocytes. Production levels were markedly elevated immediately after the treatment, peaked at 4–8 h, and had decreased to nearly the basal level by 72 h. Since the treatment promoted the release of MCP-1 in the slice cultures, but not in the enriched astrocyte cultures, it is unlikely that NMDA directly acted on the astrocytes. These results suggest that information on neuronal injury induced by NMDA is transmitted to astrocytes to induce the production of MCP-1. Organotypic slice cultures are useful for investigating the inflammatory responses of astrocytes in the process of neuronal injury.