Profile of the alpha-bungarotoxin-binding regions on the extracellular part of the alpha-chain of Torpedo californica acetylcholine receptor.
Profile of the alpha-bungarotoxin-binding regions on the extracellular part of the alpha-chain of Torpedo californica acetylcholine receptor.
复制标题
加州鱼雷乙酰胆碱受体 α 链胞外部分的 α-金环蛇毒素结合区概况。
DOI：
10.1042/bj2480847
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Atassi,MZ
中科院分区：
文献类型：
--
作者：
Mulac-Jericevic,B;Atassi,MZ
通过125I标记的α-银环蛇毒素与覆盖整个链的合成重叠肽的反应，定位了鱼雷加利福尼亚州乙酰胆碱受体α链胞外部分(残基1-210)上连续的α-神经毒素结合区。结合的特异性通过用未标记的毒素和(对于适当的多肽)未标记的抗乙酰胆碱受体抗体的抑制来证实。在残基1-10、32-41、100-115、122-150和182-198中有5个毒素结合区。第三、第四和第五(以及较小程度的第一和第二)毒素结合区与抗(乙酰胆碱受体)抗体识别的区域重叠。这五个毒素结合区可能是不同的部位，也可能是一个(或多个)部位的不同“脸”。
The continuous alpha-neurotoxin-binding regions on the extracellular part (residues 1-210) of the alpha-chain of Torpedo californica acetylcholine receptor were localized by reaction of 125I-labelled alpha-bungarotoxin with synthetic overlapping peptides spanning this entire part of the chain. The specificity of the binding was confirmed by inhibition with unlabelled toxin and, for appropriate peptides, with unlabelled anti-(acetylcholine receptor) antibodies. Five toxin-binding regions were localized within residues 1-10, 32-41, 100-115, 122-150 and 182-198. The third, fourth and fifth (and to a lesser extent the first and second) toxin-binding regions overlapped with regions recognized by anti-(acetylcholine receptor) antibodies. The five toxin-binding regions may be distinct sites or, alternatively, different ‘faces’ in one (or more) sites.