Generation of knockout rabbits using transcription activator-like effector nucleases.
Generation of knockout rabbits using transcription activator-like effector nucleases.
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DOI：
10.1186/2045-9769-3-3
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发表时间：
2014
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影响因子：
--
通讯作者：
Lai L
中科院分区：
文献类型：
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作者：
Wang Y;Fan N;Song J;Zhong J;Guo X;Tian W;Zhang Q;Cui F;Li L;Newsome PN;Frampton J;Esteban MA;Lai L
锌指核酸酶和转录激活因子样效应核酸酶是新的基因编辑平台，有助于重新定义现代生物学研究的边界。它们由非特异性切割结构域和定制的DNA结合模块组成，其通过在所需基因座处诱导有效的DNA双链断裂来实现广泛的遗传修饰。在其他显着的用途中，这些核酸酶已被用于在中型和大型动物中产生基因敲除，例如分别是兔子和猪。这种方法具有成本效益，相对快速，并且可以为人类疾病研究，生物技术或农业目的提供宝贵的模型。在这里，我们描述了一个协议，使用转录激活因子样效应核酸酶的敲除兔的有效生成，和该领域的前景。本文的在线版本（doi：10.1186/2045-9769-3-3）包含补充材料，可供授权用户使用。
Zinc-finger nucleases and transcription activator-like effector nucleases are novel gene-editing platforms contributing to redefine the boundaries of modern biological research. They are composed of a non-specific cleavage domain and a tailor made DNA-binding module, which enables a broad range of genetic modifications by inducing efficient DNA double-strand breaks at desired loci. Among other remarkable uses, these nucleases have been employed to produce gene knockouts in mid-size and large animals, such as rabbits and pigs, respectively. This approach is cost effective, relatively quick, and can produce invaluable models for human disease studies, biotechnology or agricultural purposes. Here we describe a protocol for the efficient generation of knockout rabbits using transcription activator-like effector nucleases, and a perspective of the field. The online version of this article (doi:10.1186/2045-9769-3-3) contains supplementary material, which is available to authorized users.