Cross-sensitivity to DNA-damaging agents in radiation-sensitive mutants of murine leukemia cells.
Cross-sensitivity to DNA-damaging agents in radiation-sensitive mutants of murine leukemia cells.
复制标题
鼠白血病细胞辐射敏感突变体对 DNA 损伤剂的交叉敏感性。
作者：
K. Sato;A. Ito;N. Hieda;T. Shiomi;H. Hama
比较了两个独立分离的小鼠L5178Y细胞辐射敏感突变体M10和LX830对不同DNA损伤剂的敏感性。这两个突变株对伽玛射线、博莱霉素、阿糖胞苷(Ara-C)和阿霉菌素都有超敏反应。但它们的不同之处在于，M10对甲烷磺酸甲酯(MMS)的杀灭比LX830或L5178Y更敏感，而LX830对羟基脲和3-氨基苯甲酰胺的灭活比M10或L5178Y更敏感。对丝裂霉素C(MMC)和4-硝基喹啉-L氧化物(4NQO)的反应以M10最强，L5178Y次之，LX830次之。M10和LX830之间的互补试验表明，M10-LX830杂交细胞的X射线存活曲线与M10或LX830相似。在MMS敏感的M10细胞中发现了一个MMS抗性克隆，并被证明与原始M10细胞一样对X射线杀伤敏感。这些结果表明，M10和LX830在修复X射线、博莱霉素、Ara-C和Ahidiclin引起的损伤方面有共同的缺陷，而在MMS-、MMC-、4NQO-、羟基脲和3-氨基苯甲酰胺损伤的修复方面有不同的缺陷。
Two independently isolated radiation-sensitive mutants, M10 and LX830, of mouse L5178Y cells were compared for their sensitivity to various DNA-damaging agents. These two mutants share hypersensitivity to gamma rays, bleomycin, arabinofuranosyl cytosine (Ara-C) and aphidicolin. But they differ in that M10 is more sensitive to killing by methyl methanesulfonate (MMS) than LX830 or L5178Y, whereas LX830 is more sensitive to inactivation by hydroxyurea and 3-aminobenzamide than M10 or L5178Y. The response to mitomycin C (MMC) and 4-nitroquinoline-l-oxide (4NQO) is the most pronounced in M10, the least in L5178Y and intermediate in LX830. The complementation tests between M10 and LX830 showed that the X-ray survival curves of M10-LX830 hybrid cells were similar to those of M10 or LX830. An MMS-resistant clone was found among MMS-sensitive M10 cells and proved to be just as sensitive to killing by X-rays as the original M10 cells. These results indicate that M10 and LX830 have a common defect in the repair of damage caused by X rays, bleomycin, Ara-C and aphidicolin but a different defect in the repair of MMS-, MMC-, 4NQO-, hydroxyurea and 3-aminobenzamide-damage.