Intramyocellular lipid quantification:: Repeatability with 1H MR spectroscopy
Intramyocellular lipid quantification:: Repeatability with 1H MR spectroscopy
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DOI：
10.1148/radiol.2362041661
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发表时间：
2005-08-01
期刊：
影响因子：
19.7
通讯作者：
Palmer, WE
中科院分区：
文献类型：
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作者：
Torriani, M;Thomas, BJ;Palmer, WE
目得：为了前瞻性地确定胫骨前肌内脂质（IMCL）的可重复性和可变性进行定量，通过使用1.5 T氢1（H-1）磁共振（MR）spectroscopy in healthy subjects.MATERIALS AND METHODS：机构审查委员会批准和书面知情同意书，获得了这项健康保险便携性和责任法案的研究。作者使用单体素短回波时间点分辨H-1 MR波谱检查了27名年龄在19-48岁（12名男性，15名女性;平均年龄25岁）的健康受试者的胫骨前肌。在第一次访问期间，受试者在重新定位在磁体孔中之前和之后进行H-1 MR波谱分析，体素小心地放置在骨性标志的基础上。平均间隔12天后重复测量。通过使用基于熔岩的MR用户界面（jMRUI）和LCModel软件拟合所有光谱，并将脂质峰缩放至未抑制的水峰（4.7 ppm）和总肌酸峰（约3.0 ppm）。使用单向随机效应方差分量模型确定日内和访视间变异系数（CV）。进行功率分析，以确定可检测的百分比变化，在脂质测量两个主题的samplesizes.RESULTS：测量IMCL亚甲基质子峰在1.3 ppm的共振缩放到未抑制的水峰（IMCLW），通过使用jMRUI软件获得了最低的CV整体（日内和访视间CV，分别为13.4%和14.4%）。随机效应方差分量模型显示，非生物因素（设备和重新定位）占IMCL量化总变异的50%。功率分析的样本量为20名受试者显示，IMCLW的变化大于15%，可以放心地检测到H-1 MR光谱测量获得不同day.CONCLUSION：H-1 MR光谱是可行的可重复定量IMCL浓度在纵向研究肌肉代谢。(c)RSNA，2005年
PURPOSE: To prospectively determine the repeatability and variability of tibialis anterior intramyocellular lipid (IMCL) quantifications performed by using 1.5-T hydrogen 1(H-1) magnetic resonance (MR) spectroscopy in healthy subjects.MATERIALS AND METHODS: Institutional review board approval and written informed consent were obtained for this Health Insurance Portability and Accountability Act-compliant study. The authors examined the anterior tibial muscles of 27 healthy subjects aged 19-48 years (12 men, 15 women; mean age, 25 years) by using single-voxel short-echo-time point-resolved H-1 MR spectroscopy. During a first visit, the subjects underwent H-1 MR spectroscopy before and after being repositioned in the magnet bore, with voxels carefully placed on the basis of osseous landmarks. Measurements were repeated after a mean interval of 12 days. All spectra were fitted by using lava-based MR user interface (jMRUI) and LCModel software, and lipid peaks were scaled to the unsuppressed water peak (at 4.7 ppm) and the total creatine peak (at approximately 3.0 ppm). A one-way random-effects variance components model was used to determine intraday and intervisit coefficients of variation (CVs). A power analysis was performed to determine the detectable percentage change in lipid measurements for two subject sample sizes.RESULTS: Measurements of the IMCL methylene protons peak at a resonance of 1.3 ppm scaled to the unsuppressed water peak (IMCLW) that were obtained by using jMRUI software yielded the lowest CVs overall (intraday and intervisit CVs, 13.4% and 14.4%, respectively). The random-effects variance components model revealed that nonbiologic factors (equipment and repositioning) accounted for 50% of the total variability in IMCL quantifications. Power analysis for a sample size of 20 subjects revealed that changes in IMCLW of greater than 15% could be confidently detected between H-1 MR spectroscopic measurements obtained on different days.CONCLUSION: H-1 MR spectroscopy is feasible for repeatable quantification of IMCL concentrations in longitudinal studies of muscle metabolism. (c) RSNA, 2005