Preparation and biological evaluation of a novel agarose-grafting-hyaluronan scaffold for accelerated wound regeneration
Preparation and biological evaluation of a novel agarose-grafting-hyaluronan scaffold for accelerated wound regeneration
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DOI：
10.1088/1748-605x/ab7b3e
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发表时间：
2020-07-01
影响因子：
4
通讯作者：
Tang, Shunqing
中科院分区：
文献类型：
--
作者：
Chu, Bin;Zhang, Ailian;Tang, Shunqing
目前，寻求一种有效的创面再生敷料已引起了人们的极大兴趣。本文合成了一种新的琼脂糖接枝透明质酸（Ag-g-HA）支架，用于快速伤口愈合。元素分析结果表明，Ag-g-HA的HA接枝率接近69%。Ag-g-HA保持生物活性，以加速细胞增殖并刺激巨噬细胞RAW 264.7分泌TNF-α，以及成纤维细胞3T3分泌胶原蛋白I和胶原蛋白III。一项体内研究表明，Ag-g-HA对全层皮肤缺损显示出更快的修复周期和更好的皮肤组织学结构。Ag-g-HA小鼠分泌的I型胶原、III型胶原和TNF-α与HA相似。Ag-g-HA具有与HA相似的生物学活性，但由于其不溶性的提高，其降解时间延长。这些结果表明，Ag-g-HA支架加速伤口愈合，并可能是一个有前途的新型支架的组织工程和再生医学。
At present, seeking an effective dressing for wound regeneration has drawn considerable interest. In this paper, a novel agarose-grafting-hyaluronan (Ag-g-HA) scaffold was synthesized for rapid wound healing. Elemental analysis results showed that the HA grafting rate of Ag-g-HA was similar to 69%. Ag-g-HA remained bioactive to accelerate cell proliferation and stimulate secretion of TNF-alpha for macrophagocyte RAW 264.7, and collagen I and collagen III for fibroblast 3T3. An in vivo study demonstrated that Ag-g-HA showed a faster repair cycle and a better skin histological structure for a full-thickness skin defect. The collagen I, collagen III and TNF-alpha secreted by mice for Ag-g-HA were similiar to HA. Ag-g-HA showed a similiar biological activity to HA but had a longer degradation time through its improved insolubility. These findings demonstrate that the Ag-g-HA scaffold accelerated wound healing, and could be a promising novel scaffold for tissue engineering and regenerative medicine.