Systemin triggers an increase of cytoplasmic calcium in tomato mesophyll cells: Ca2+ mobilization from intra‐ and extracellular compartments
Systemin triggers an increase of cytoplasmic calcium in tomato mesophyll cells: Ca2+ mobilization from intra‐ and extracellular compartments
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系统素引发番茄叶肉细胞中细胞质钙的增加：细胞内和细胞外的 Ca2+ 动员
DOI：
10.1046/j.1365-3040.1998.00378.x
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发表时间：
1998
影响因子：
7.3
通讯作者：
E. Johannes
中科院分区：
文献类型：
--
作者：
C. Moyen;K. Hammond;J. Jones;M. Knight;E. Johannes
我们在这里表明，在施用1-2分钟内，系统素触发了番茄叶肉细胞胞质游离钙浓度（[ca2 +] c）的短暂增加。l型ca2 +通道阻滞剂（硝苯地平、维拉帕米和地尔硫卓）和ca2 +螯合剂[乙二醇四乙酸（EGTA）]能轻微但不显著地降低系统诱导的ca2 +升高，而无机ca2 +通道阻滞剂（lacl3）则能显著降低系统诱导的ca2 +升高。cdcl2和gdcl3)和影响胞内ca2 +从液泡释放的化合物（钌红、LiCI、新霉素）强烈地降低了系统诱导的[ca2 +] c的增加。相比之下，钾和氯通道阻滞剂没有抑制作用。与系统素不同，其他蛋白酶抑制剂（PI）和伤口诱导蛋白合成的诱导剂，如茉莉酸（JA）和贝斯特汀，不会触发细胞质ca2 +的增加。系统素诱导的细胞质ca2 +的升高可能是系统素信号通路的早期步骤，似乎涉及细胞外ca2 +通过几种类型的ca2 +可渗透通道同时流入。细胞内ca2 +的释放对肌醇1,4,5-三磷酸(ip3)-和环腺苷5'-二磷酸核糖(cADPR)-介导的ca2 +释放阻断剂敏感。
We show here that, within 1-2 min of application, systemin triggers a transient increase of cytoplasmic free calcium concentration ([Ca 2+ ] c ) in cells from Lycopersicon esculentum mesophyll. The systemin-induced Ca 2+ increase was slightly but not significantly reduced by L-type Ca 2+ channel blockers (nifedipine, verapamil and diltiazem) and the Ca 2+ chelator [ethylene glycol tetraacetic acid (EGTA)], whereas inorganic Ca 2+ channel blockers (LaCl 3 . CdCl 2 and GdCl 3 ) and compounds affecting the release of intracellular Ca 2+ from the vacuole (ruthenium red, LiCI, neomycin) strongly reduced the systemin-induced [Ca 2+ ] c increase. By contrast, no inhibitory effect was seen with the potassium and chloride channel blockers tested. Unlike systemin, other inducers of proteinase inhibitor (PI) and of wound-induced protein synthesis, such as jasmonic acid (JA) and bestatin, did not trigger an increase of cytoplasmic Ca 2+ , The systemin-induced elevation of cytoplasmic Ca 2+ which might be an early step in the systemin signalling pathway, appears to involve an influx of extracellular Ca 2+ simultaneously through several types of Ca 2+ permeable channels, and a release of Ca 2+ from intracellular stores sensitive to blockers of inositol 1,4,5-triphosphate (IP 3 )- and cyclic adenasine 5'-diphosphoribose (cADPR)-mediated Ca 2+ release.
DOI：
10.1073/pnas.93.22.12053
发表时间：
1996-10-29
影响因子：
11.1
作者：
Bergey, DR;Howe, GA;Ryan, CA
通讯作者：
Ryan, CA