LIPOPROTEIN-LIPASE REGULATION BY INSULIN AND GLUCOCORTICOID IN SUBCUTANEOUS AND OMENTAL ADIPOSE TISSUES OF OBESE WOMEN AND MEN
LIPOPROTEIN-LIPASE REGULATION BY INSULIN AND GLUCOCORTICOID IN SUBCUTANEOUS AND OMENTAL ADIPOSE TISSUES OF OBESE WOMEN AND MEN
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DOI：
10.1172/jci116821
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发表时间：
1993-11-01
影响因子：
15.9
通讯作者：
BROLIN, RE
中科院分区：
文献类型：
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作者：
FRIED, SK;RUSSELL, CD;BROLIN, RE
脂蛋白脂酶(LPL)活性在不同脂肪库之间存在显著差异，尤其是在女性中。与LPL活性的数据一致，LPL mRNA在大网膜(OM)的表达水平低于皮下(SQ)脂肪组织。为了研究这些差异的细胞学基础，将手术中从肥胖男性和女性获得的OM和SQ脂肪组织置于器官培养7d，并加入不同浓度的胰岛素和地塞米松。胰岛素可增加腹部SQ脂肪组织LPL mRNA水平和LPL活性，但不能增加OM脂肪组织中LPL的活性。地塞米松也增加了LPL的mRNA和LPL的活性，这些影响在OM脂肪组织中更加明显，尤其是在男性。当胰岛素和地塞米松一起使用时，两个储存库的LPL活性都出现了协同增加，这在一定程度上是从LPL mRNA的水平上解释的。在胰岛素存在的情况下，SQ库对次最大剂量地塞米松的影响更敏感。胰岛素或胰岛素+地塞米松诱导的LPL在SQ的最大活性高于OM库，这与LPL的mRNA水平升高有关。LPL合成速率与LPL mRNA水平呈平行关系。这些数据表明，胰岛素和糖皮质激素在LPL基因表达水平和翻译后水平上影响人类脂肪组织LPL活性，并且对这些激素影响的反应取决于脂肪储备和性别。
There are marked variations in the activity of lipoprotein lipase (LPL) among adipose depots, particularly in women. Consistent with data on LPL activity, the level of expression of LPL mRNA was lower in omental (OM) than subcutaneous (SQ) adipose tissue of women. To investigate the cellular basis of these differences, OM and SQ adipose tissues obtained at surgery from obese men and women were placed in organ culture for 7 d with varying concentrations of insulin and dexamethasone. Insulin increased levels of LPL mRNA and LPL activity in abdominal SQ but not OM adipose tissue. Dexamethasone also increased LPL mRNA and LPL activity, and these effects were more marked in the OM adipose tissue, particularly in men. When insulin and dexamethasone were added together, synergistic increases in LPL activity were seen in both depots, and this was in part explained at the level of LPL mRNA. The SQ depot was more sensitive to the effects of submaximal doses of dexamethasone in the presence of insulin. The maximum activity of LPL induced by insulin or insulin plus dexamethasone was higher in the SQ than in the OM depot of women, and this was associated with higher levels of LPL mRNA. Rates of LPL synthesis paralleled LPL mRNA levels. These data show that insulin and glucocorticoids influence human adipose tissue LPL activity at the level of LPL gene expression, as well as posttranslationally, and that responsiveness to these hormonal effects is dependent on adipose depot and gender.