Isolation and characterization of a stress-inducible Dunaliella salina Lcy-β gene encoding a functional lycopene β-cyclase
Isolation and characterization of a stress-inducible Dunaliella salina Lcy-β gene encoding a functional lycopene β-cyclase
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DOI：
10.1007/s00253-008-1492-4
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发表时间：
2008-07-01
影响因子：
5
通讯作者：
Varela, Joao
中科院分区：
文献类型：
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作者：
Ramos, Ana;Coesel, Sacha;Varela, Joao
耐盐的绿色盐藻在各种胁迫条件下积累大量的β-胡萝卜素。虽然已经发表了一些关于β-胡萝卜素的积累和生物技术生产的研究，但D.盐沼仍然鲜为人知。本文报道了番茄红素β-环化酶（Lcy-β）基因的分离及其在非生物胁迫下的表达调控。该基因在大肠杆菌中的功能是通过异源遗传互补来确定的。杆菌基因表达和生理分析表明，D.盐藻Lcy-β稳态转录物和类胡萝卜素水平响应于测试的所有胁迫条件（盐、光和营养物耗尽）而上调。研究结果表明，养分有效性是影响胡萝卜素合成及类胡萝卜素生物合成相关基因表达的关键因素。salina。
The halotolerant green alga Dunaliella salina accumulates large amounts of beta-carotene when exposed to various stress conditions. Although several studies concerning accumulation and biotechnological production of beta-carotene have been published, the molecular basis and regulation of the genes involved in carotenoid biosynthesis in D. salina are still poorly known. In this paper, we report the isolation and regulation of the lycopene beta-cyclase (Lcy-beta) gene by abiotic stress. The function of this gene was determined by heterologous genetic complementation in E. coli. Gene expression and physiological analyses revealed that D. salina Lcy-beta steady-state transcript and carotenoid levels were up-regulated in response to all stress conditions tested (salt, light and nutrient depletion). The results presented here suggest that nutrient availability is a key factor influencing carotenogenesis as well as carotenoid biosynthesis-related gene expression in D. salina.