High-Throughput Screening of Tyrosine Kinase Inhibitor Resistant Genes in CML.
High-Throughput Screening of Tyrosine Kinase Inhibitor Resistant Genes in CML.
复制标题
CML 酪氨酸激酶抑制剂抗性基因的高通量筛选。
DOI：
10.1007/978-1-4939-4011-0_14
复制
发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Green,MichaelR
中科院分区：
文献类型：
--
作者：
Ma,Leyuan;Roderick,Justine;Kelliher,MichelleA;Green,MichaelR
哺乳动物细胞全基因组RNA干扰（RNAi）筛选已被证明是识别与许多细胞过程和疾病相关的新基因和分子途径的有力工具。例如，筛选失活时导致对癌症治疗药物产生耐药性的基因可以揭示耐药性如何产生的新机制，并确定克服耐药性的潜在靶向策略。在这里，我们描述了使用全基因组人类短发夹RNA （shRNA）文库进行高通量RNAi筛选的详细程序，以鉴定人类CML细胞系模型中的酪氨酸激酶抑制剂（TKI）抗性基因。
Genome-wide RNA interference (RNAi) screening in mammalian cells has proven to be a powerful tool for identifying new genes and molecular pathways relevant to many cellular processes and diseases. For example, screening for genes that, when inactivated, lead to resistance to cancer therapeutic drugs can reveal new mechanisms for how resistance develops and identify potential targetable strategies to overcome drug resistance. Here, we describe a detailed procedure for performing a high-throughput RNAi screen using a genome-wide human short hairpin RNA (shRNA) library for identifying tyrosine kinase inhibitor (TKI)-resistance genes in a human CML cell line model.