Accumulation of some hexoses in adipocytes: properties of the system.
Accumulation of some hexoses in adipocytes: properties of the system.
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脂肪细胞中一些己糖的积累：系统的特性。
DOI：
10.1152/ajpcell.1989.257.2.c214
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Kleinzeller,A
中科院分区：
文献类型：
--
作者：
Thompson,KA;Kleinzeller,A
脂肪细胞积累2-脱氧-d -葡萄糖（2-DG）具有显著的化学梯度（J. Foley和J. Gliemann）。Biochim。Biophys。学报（英文版）：100-106,1981。研究了这种积累过程的特异性。分离的大鼠脂肪细胞在37℃下与0.1 mM己糖孵育，积累游离2-脱氧-d -半乳糖（2-DGal）或d -半乳糖（Gal），稳态梯度分别为5.5 +/- 1.2和2.5 +/- 0.7。像2-DG一样，2-DGal和Gal都是磷酸化的底物。胰岛素和胰岛素模拟药物增加了Gal、2-DGal、游离和磷酸化2-DG的稳态积累（100 nM胰岛素使游离2-DG从2.1 +/- 0.2 mM增加到6.6 +/- 0.3 mM；外用2-DG = 0.1 mM）。去除细胞外钙或钠或存在A23187或瓦巴因未能抑制2-DG的积累。洋地黄苷或高压放电诱导的质膜透性导致细胞2-DG和2-DG-磷酸的损失，但不影响3- o -甲基- d -葡萄糖的平衡。数据表明，无论是跨膜离子梯度还是细胞内区隔都不足以解释积累过程的机制。讨论了磷酸化在己糖积累过程中可能起的作用。
Adipocytes accumulate 2-deoxy-D-glucose (2-DG) against significant chemical gradients (J. Foley and J. Gliemann. Biochim. Biophys. Acta 648: 100-106, 1981). The specificity of this accumulation process was examined. Isolated rat adipocytes incubated at 37 degrees C with 0.1 mM hexoses accumulated free 2-deoxy-D-galactose (2-DGal) or D-galactose (Gal) against steady-state gradients of 5.5 +/- 1.2 and 2.5 +/- 0.7, respectively. Like 2-DG, both 2-DGal and Gal are substrates for phosphorylation. Insulin and insulin-mimetic agents increased steady-state accumulations of Gal, 2-DGal, and free and phosphorylated 2-DG (100 nM insulin increased free 2-DG from 2.1 +/- 0.2 to 6.6 +/- 0.3 mM; external 2-DG = 0.1 mM). Removal of extracellular calcium or sodium or the presence of A23187 or ouabain failed to inhibit 2-DG accumulation. Plasma membrane permeabilization induced by either digitonin or high-voltage discharge produced a loss of cellular 2-DG and 2-DG-phosphate without affecting 3-O-methyl-D-glucose equilibration. The data indicate that neither transmembrane ionic gradients nor intracellular compartmentation suffice as explanations of the mechanism of the accumulation process. The possible role of phosphorylation in the process of hexose accumulation is discussed.