Molecular Phylogenetic Analysis of Ribosomal DNA Internal Transcribed Spacer Regions and Comparison of Fertility in Phomopsis Isolates from Fruit Trees
Molecular Phylogenetic Analysis of Ribosomal DNA Internal Transcribed Spacer Regions and Comparison of Fertility in Phomopsis Isolates from Fruit Trees
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果树拟茎点霉分离物核糖体 DNA 内转录间隔区的分子系统发育分析及繁殖力比较
DOI：
10.1007/pl00012944
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发表时间：
2000
影响因子：
1.2
通讯作者：
Y. Ohtsu
中科院分区：
文献类型：
--
作者：
S. Kanematsu;N. Minaka;Takao Kobayashi;A. Kudō;Y. Ohtsu
检查核糖体 DNA (rDNA) 内转录间隔区 (ITS) 区域的序列，以推断小孢子拟茎点霉的分子系统发育，指定为 W 型（主要为白色菌落，弱毒力，在 25°C 下 PDA 上同时带有 α 和 β 分生孢子）和 G 型（主要为灰色菌落，高毒力，在 25°C 下仅带有 α 分生孢子） PDA) 和 P.杏仁来自果树。拟茎点霉G型和P型。杏仁核是一个与 W 型不同的单系群。 W型分离株分为两个单系类群：Diaporthe citri、D. tanakae、P. asparagi、P. viticola、P. vitimegaspora 和 D. asparagi。 nomurai 在形态上与 W 型和 G 型有区别，在分子系统发育分析中与 W 型和 G 型不同。 rDNA ITS 区域的 PCR-RFLP 分析可用于使用三种限制性内切酶区分每个拟茎点霉属物种和群体。在交配试验中，来自果树的 W 型分离株是异宗合的，并且即使在属于不同单系群的分离株之间也是可育的。 G型和P型的分离株。在日本收集的杏仁核是交叉受精的。来自青月草、光榆和核桃的一些分离株属于W型的两个单系类群之一，并且与来自蔷薇科果树的W型分离株杂交可育。
Sequences of the ribosomal DNA (rDNA) internal transcribed spacer (ITS) regions were examined to infer a molecular phylogeny of small-sporedPhomopsisisolates, designated W-type (mainly white colony, weakly virulent, bearing both alpha and beta conidia at 25°C on PDA) and G-type (mainly gray colony, highly virulent, bearing only alpha conidia at 25°C on PDA), andP. amygdalifrom fruit trees.PhomopsisG-type andP. amygdaliwere a monophyletic group distinct from the W-type. The W-type isolates were divided into two monophyletic groups.Diaporthe citri, D. tanakae, P. asparagi, P. viticola, P. vitimegasporaandD. nomurai,which are morphologically distinguishable from W- and G-types, differed from the W- and G-types in molecular phylogenetic analyses. PCR-RFLP analysis of rDNA ITS regions was useful to distinguish each of thePhomopsisspecies and groups using three restriction enzymes. In mating tests, W-type isolates from fruit trees were heterothallic and inter-fertile even between isolates belonging to the different monophyletic groups. Isolates of the G-type andP. amygdalicollected in Japan were cross-fertile. Some isolates fromLunaria annua, Ulmus glabraandJuglans regiabelonged to one of the two monophyletic groups of the W-type and were cross-fertile with W-type isolates from Rosaceous fruit trees.