Frequency of immunoglobulin E class switching is autonomously determined and independent of prior switching to other classes.
Frequency of immunoglobulin E class switching is autonomously determined and independent of prior switching to other classes.
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DOI：
10.1084/jem.179.6.2023
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发表时间：
1994-06-01
影响因子：
15.3
通讯作者：
Radbruch, A
中科院分区：
文献类型：
--
作者：
Jung, S;Siebenkotten, G;Radbruch, A
在人类和小鼠中，表达免疫球蛋白E（IgE）的B淋巴细胞的主要部分通过从IgM经IgG到IgE的顺序IG类别转换而形成。这种顺序的类别转换可能对IgE+细胞的频率和库具有功能性影响。在这里，我们表明，在突变小鼠中，通过IgG1连续切换到IgE被阻断，细胞切换到IgE的频率不受影响。因此，顺序类别转换为IgE仅仅反映了两个受体开关区域的开关重组的同时可及性，由一种细胞因子诱导，但具有明显不同的效率。开关重组的开关细胞上的两个IgH等位基因的分析表明，低频率的切换到IgE是一个固有的特征的S开关区域及其控制元件。
Both, in humans and in mice, a major fraction of immunoglobulin E (IgE)- expressing B lymphocytes develops by sequential Ig class switching from IgM via IgG to IgE. This sequential class switch might have functional implications for the frequency and repertoire of IgE+ cells. Here we show that in mutant mice, in which sequential switching to IgE via IgG1 is blocked, the frequency of cells switching to IgE is not affected. Thus, sequential class switching to IgE merely reflects the simultaneous accessibility of two acceptor switch regions for switch recombination, induced by one cytokine, but with markedly distinct efficiency. Analysis of switch recombination on both IgH alleles of switched cells shows that the low frequency of switching to IgE is an inherent feature of the S epsilon switch region and its control elements.