Effects of UV/H2O2 degradation and step gradient ethanol precipitation on Sargassum fusiforme polysaccharides: Physicochemical characterization and protective effects against intestinal epithelial injury.
Effects of UV/H2O2 degradation and step gradient ethanol precipitation on Sargassum fusiforme polysaccharides: Physicochemical characterization and protective effects against intestinal epithelial injury.
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DOI：
10.1016/j.foodres.2022.111093
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发表时间：
2022-03
影响因子：
8.1
通讯作者：
Wanzi Yao;Mengyuan Liu;Xiaoyong Chen;Lijun You;Yongxuan Ma;K. Hileuskaya
中科院分区：
文献类型：
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作者：
Wanzi Yao;Mengyuan Liu;Xiaoyong Chen;Lijun You;Yongxuan Ma;K. Hileuskaya
采用紫外线/过氧化氢（UV/H2 O2）降解法和乙醇分级沉淀法对羊栖菜多糖（Sargassum fusiformes polysaccharides，SFP）进行降解纯化，得到纯化组分DSFP。结果表明，处理显着降低多糖的分子量，从282.83 kDa至18.54 kDa，并影响其表面形态和粗糙度。SFP和DSFP是典型的硫酸多糖，主要由岩藻糖、半乳糖醛酸、葡萄糖醛酸、半乳糖和甘露糖组成。SFP和DSFP均能促进大鼠肠上皮细胞（IEC-6）在创伤愈合过程中的细胞迁移，并通过促进细胞从G 0/G1期向S期的转变而刺激细胞周期进程。但DSFP对创面愈合和细胞迁移的促进作用强于SFP。增强肠道屏障功能，减轻脂多糖诱导的肠道炎症。与模型组相比，DSFP组Toll样受体4、肿瘤坏死因子-α、白细胞介素-6、白细胞介素-1 β和诱导型一氧化氮合酶的表达分别下降53.14%、92.41%、66.01%、68.24%和78.09%，白细胞介素-10的表达上调2.48倍。因此，UV/H2 O2降解和梯度乙醇沉淀处理可有效提高肠上皮细胞的保护作用。
In this study, the degraded purified fraction fromSargassum fusiformepolysaccharides (SFP), named DSFP, was produced by the treatment of ultraviolet/hydrogen peroxide (UV/H2O2) degradation and step gradient ethanol precipitation. Results showed that the treatment significantly reduced the molecular weight of polysaccharides, from 282.83 kDa to 18.54 kDa, and influenced their surface morphology and roughness. SFP and DSFP were typical sulfated polysaccharides, mainly composed of fucose, galacturonic acid, glucuronic acid, galactose, and mannose. Both SFP and DSFP increased cell migration during intestinal epithelial wound healing and stimulated the cell cycle progression by promoting the transition from G0/G1 to S phase in the rat intestine epithelium cells (IEC-6). But DSFP had a stronger positive effect on wound healing and cell migration than SFP. It reinforced the intestinal barrier function and attenuated lipopolysaccharides-induced intestinal inflammation. DSFP significantly downregulated the expression ofToll-like receptor 4,tumor necrosis factor-α,interleukin-6,interleukin-1β, andinducible nitric oxide synthaseby 53.14%, 92.41%, 66.01%, 68.24%, and 78.09%, respectively, and upregulated that ofinterleukin-10by 2.48 folds when compared to the model. Therefore, the treatment (UV/H2O2degradation and step gradient ethanol precipitation) could effectively improve the protective effects against intestinal epithelial injury.