Priming of Production in Maize of Volatile Organic Defence Compounds by the Natural Plant Activator cis-Jasmone.
Priming of Production in Maize of Volatile Organic Defence Compounds by the Natural Plant Activator cis-Jasmone.
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DOI：
10.1371/journal.pone.0062299
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Birkett MA
中科院分区：
文献类型：
--
作者：
Oluwafemi S;Dewhirst SY;Veyrat N;Powers S;Bruce TJ;Caulfield JC;Pickett JA;Birkett MA
顺式茉莉酮（CJ）是一种天然植物产品，在模型和作物植物中激活对食草动物的防御。在这项研究中，我们调查了CJ是否可以引发防御玉米，玉米，对叶蝉，Cicadulina storeyi，负责玉米条纹病毒（MSV）的传播。引发发生在预处理时，在这种情况下，CJ增加了随后攻击植物时防御反应的效力和速度。在这里，我们测试了昆虫对植物挥发性有机化合物（VOCs）的反应，使用Y型管嗅觉仪生物测定。我们的初步实验表明，在这个系统中，有没有显着的反应的食草动物CJ本身，并没有差异，从未暴露的植物收集的挥发性有机化合物的反应相比，CJ暴露的植物，都没有昆虫。然后从C收集VOC。用和不用CJ预处理的被storeyi侵染的玉米幼苗。生物测定结果表明，这种害虫对未经CJ预处理的受侵染幼苗中的VOC有明显的偏好。一个定时系列的VOC收集和生物测定表明，效果是最强的，在第一个22小时的昆虫侵扰，即之前的昆虫已经引起了变化的VOC排放。化学分析表明，玉米幼苗经CJ处理后，再经C. storeyi，导致防御性倍半萜烯（E）-（1 R，9 S）-香芹烯、（E）-α-香柠檬烯、（E）-β-法呢烯和（E）-4，8-二甲基-1，3，7-壬三烯的释放显著增加，已知这些物质用作食草动物驱避剂。化学分析解释了在嗅觉计中观察到的行为效应，因为CJ处理导致植物在昆虫侵染的前22小时内比对照植物释放出包含更多驱避剂组分的VOC混合物。CJ预处理提高了挥发性有机物的释放速度和强度。这是第一个表明，CJ可以总理植物的防御性VOCs拮抗剂对草食动物的生产增强。
cis-Jasmone (CJ) is a natural plant product that activates defence against herbivores in model and crop plants. In this study, we investigated whether CJ could prime defence in maize, Zea mays, against the leafhopper, Cicadulina storeyi, responsible for the transmission of maize streak virus (MSV). Priming occurs when a pre-treatment, in this case CJ, increases the potency and speed of a defence response upon subsequent attack on the plant. Here, we tested insect responses to plant volatile organic compounds (VOCs) using a Y-tube olfactometer bioassay. Our initial experiments showed that, in this system, there was no significant response of the herbivore to CJ itself and no difference in response to VOCs collected from unexposed plants compared to CJ exposed plants, both without insects. VOCs were then collected from C. storeyi-infested maize seedlings with and without CJ pre-treatment. The bioassay revealed a significant preference by this pest for VOCs from infested seedlings without the CJ pre-treatment. A timed series of VOC collections and bioassays showed that the effect was strongest in the first 22 h of insect infestation, i.e. before the insects had themselves induced a change in VOC emission. Chemical analysis showed that treatment of maize seedlings with CJ, followed by exposure to C. storeyi, led to a significant increase in emission of the defensive sesquiterpenes (E)-(1R,9S)-caryophyllene, (E)-α-bergamotene, (E)-β-farnesene and (E)-4,8-dimethyl-1,3,7-nonatriene, known to act as herbivore repellents. The chemical analysis explains the behavioural effects observed in the olfactometer, as the CJ treatment caused plants to emit a blend of VOCs comprising more of the repellent components in the first 22 h of insect infestation than control plants. The speed and potency of VOC emission was increased by the CJ pre-treatment. This is the first indication that CJ can prime plants for enhanced production of defensive VOCs antagonist towards herbivores.
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影响因子：
5.5
作者：
Kost, C;Heil, M
通讯作者：
Heil, M
DOI：
10.1073/pnas.1009975107
发表时间：
2010-12-07
影响因子：
11.1
作者：
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作者：
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影响因子：
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作者：
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