Bile secretion in rats with indomethacin-induced intestinal inflammation
Bile secretion in rats with indomethacin-induced intestinal inflammation
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吲哚美辛诱导肠道炎症大鼠的胆汁分泌
DOI：
10.1152/ajpgi.1996.270.5.g804
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发表时间：
1996
影响因子：
4.5
通讯作者：
T. Takeuchi
中科院分区：
文献类型：
--
作者：
Tamaki Yamada;M. Hoshino;T. Hayakawa;Y. Kamiya;H. Ohhara;K. Mizuno;H. Yamada;T. Nakazawa;T. Inagaki;A. Uchida;M. Miyaji;T. Takeuchi
本研究的目的是表征吲哚美辛诱导的亚急性肠道炎症大鼠模型的胆汁分泌，包括胆胆酸的组成、胆盐池的大小和经细胞囊泡运输。吲哚美辛治疗显著降低了胆汁酸非依赖性胆汁流量和胆汁酸和胆固醇的胆道分泌，同时增加了体内胆道磷脂的输出。虽然吲哚美辛治疗没有改变体内胆盐池的大小，但α -和β -胆酸明显减少，而胆汁中的羟基脱氧胆酸和脱氧胆酸增加。吲哚美辛处理的大鼠离体灌注肝脏的胆汁流量和牛磺胆酸运输最大值没有减少，胆汁辣根过氧化物酶的输出量显著增加。用吲哚美辛治疗的大鼠门静脉血中内毒素明显增加。克林霉素能轻微减轻肠道炎症，但能显著防止胆汁流量、胆汁酸输出和牛磺胆酸运输的减少。我们认为，虽然体内胆分泌功能明显下降，但肝细胞功能保持不变。
The objective of this study was to characterize the bile secretion, including the composition of biliary bile acids, bile salt pool size, and transcytotic vesicle transport, in a rat model of subacute intestinal inflammation induced by indomethacin. Indomethacin treatment significantly decreased bile acid-independent bile flow and biliary secretion of bile acid and cholesterol, while increasing biliary phospholipid output in vivo. Although indomethacin treatment did not change the bile salt pool size in vivo, alpha- and beta-muricholic acids were significantly deceased and hyodeoxycholic and deoxycholic acids were increased in bile. Bile flow and the transport maximum of taurocholate did not decrease, and biliary horseradish peroxidase output was significantly enhanced in isolated perfused livers from indomethacin-treated rats. Endotoxin in the portal blood was significantly increased in rats treated with indomethacin. Clindamycin slightly reduced intestinal inflammation but significantly prevented decreases in bile flow, bile acid output, and transport maximum of taurocholate. We conclude that, although biliary secretory function was apparently decreased in vivo, that of hepatocyte function was maintained in this model.