Colloidal Palladium Particles of Different Shapes for Electron Microscopy Labeling
Colloidal Palladium Particles of Different Shapes for Electron Microscopy Labeling
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DOI：
10.1017/s1431927609991188
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发表时间：
2010-02-01
影响因子：
2.8
通讯作者：
Albrecht, Ralph M.
中科院分区：
文献类型：
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作者：
Meyer, Daryl A.;Oliver, Julie A.;Albrecht, Ralph M.
免疫金技术是标记细胞大分子的一种有价值的方法。然而，使用不同大小的胶体金(CAU)纳米粒子进行多重标记存在一定的缺陷；即，随着粒子尺寸的增加，标记效率降低，并且相对于标记表位的位置的空间分辨率降低。这两个问题也限制了重金属颗粒在标记密度比较分析中的应用。为了最大限度地减少由于标记效率不同而产生的变量，最好的解决方案是使用大小相似的颗粒进行多次标记。因此，除了大小之外，还需要一些参数来区分每种标签类型。在这项研究中，我们报道了尺寸相似但具有两种不同形状的胶体钯(CPD)纳米颗粒的合成，它们是碳酸盐和多面体，很容易与球形胶体金颗粒区分开来。它们的实用性和保真度作为标签使用人类血小板整体安装模型也得到了演示。
The immunogold technique is a valuable method for labeling cellular macromolecules. However, multiple labeling using colloidal gold (cAu) nanoparticles of different sizes presents certain drawbacks; namely, as particle size increases, there is a decreased labeling efficiency and diminished spatial resolution with respect to the locations of labeled epitopes. Both concerns also limit the utility of heavy metal particles for comparative analysis of labeling densities. To minimize the variables due to differential labeling efficiencies, the best Solution Would be to conduct multiple labeling with particles of similar size. Consequently, some parameter other than size is necessary to distinguish each label type. In this study, we report the synthesis of colloidal palladium (cPd) nanoparticles of similar size but having two distinct shapes, umbonate and faceted, which are readily distinguishable from spherical colloidal gold particles. Their utility and Fidelity as labels using a human platelet whole-mount model is also demonstrated.