Repetitive domestication to enhance butanol tolerance and production in Clostridium acetobutylicum through artificial simulation of bio-evolution
Repetitive domestication to enhance butanol tolerance and production in Clostridium acetobutylicum through artificial simulation of bio-evolution
复制标题
通过生物进化的人工模拟重复驯化以增强丙酮丁醇梭菌的丁醇耐受性和产量
DOI：
10.1016/j.biortech.2012.12.121
复制
发表时间：
2013-02-01
影响因子：
11.4
通讯作者：
Yu, Xiao-Bin
中科院分区：
文献类型：
--
作者：
Liu, Xiao-Bo;Gu, Qiu-Ya;Yu, Xiao-Bin
为了提高乙酰丁酸梭菌（Clostridium acetobutylicum）对丁醇的耐受性和产量，本研究提出了一种基于进化动力学和自然选择的生物进化人工模拟（ASBE）方法。通过反复进化驯化，获得了一株耐丁醇菌株C. acetobutylicum T64，该菌株能耐4% （v/v）丁醇（野生型为2%），以玉米粉为底物，丁醇产量从12.2 g/L提高到15.3 g/L。通过发酵研究丁醇耐受性与ABE产量之间的关系，结果表明提高丁醇耐受性可以增加丁醇产量，但不能提高ABE的总产量。这些结果也表明ASBE将是一种可行的生物技术方法，用于提高丁醇耐受性和产量。(C) 2012 Elsevier Ltd.版权所有。
To improve butanol tolerance and production in Clostridium acetobutylicum, a novel approach was developed in this study, which was called artificial simulation of bio-evolution (ASBE) based on the evolutionary dynamics and natural selection. Through repetitive evolutionary domestications, a butanol-tolerant strain C. acetobutylicum T64 was obtained, which could withstand 4% (v/v) (compared to 2% of the wildtype) butanol and was accompanied by the increase of butanol production from 12.2 g/L to 15.3 g/L using corn meal as substrate. Fermentation was also carried out to investigate the relationship between butanol tolerance and ABE production, suggesting that enhancing butanol tolerance could increase butanol production but unlikely improve total ABE production. These results also indicated that the ASBE would be an available and feasible method used in biotechnology for enhancement of butanol tolerance and production. (C) 2012 Elsevier Ltd. All rights reserved.