Spatial and temporal requirement of Defective proventriculus activity during Drosophila midgut development
Spatial and temporal requirement of Defective proventriculus activity during Drosophila midgut development
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DOI：
10.1016/j.mod.2011.02.003
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发表时间：
2011-05-01
影响因子：
2.6
通讯作者：
Nakagoshi, Hideki
中科院分区：
文献类型：
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作者：
Nakagawa, Yoshiki;Fujiwara-Fukuta, Shinobu;Nakagoshi, Hideki
果蝇中肠细胞起源于内胚层，发育为四种不同类型的细胞。在这些细胞中，铜细胞在其顶端表面上具有内陷的微绒毛膜，并且它们参与两种不同的功能，即，铜吸收和酸分泌。同源异型盒基因缺陷前胃（dve）在中肠中表达，并且已经鉴定了两种转录物，A型（类似于4.9 kb）和B型（类似于3.5 kb）。我们分离了完全去除A型（dve-A）转录本第一个外显子的缺失等位基因dve（E181）。在dve-A突变体背景中的Dve表达模式表明同种型转换以细胞类型特异性方式动态调节。使用RNAi的dve-A，我们检查的Dve-A活性的空间和时间的要求。需要早期Dve-A活性来抑制铜细胞中的同种型转换，并建立两种肠道功能。晚期Dve-A活性在铜细胞中，但不是在相邻的间质细胞中，需要酸分泌，而活动是多余的铜吸收功能所需的两个细胞。此外，异位B型表达在幼虫铜细胞特异性损害铜吸收功能。这些结果为建立功能特异性的分子机制提供了见解。(C)2011爱思唯尔爱尔兰有限公司保留所有权利。
The Drosophila middle midgut cells derived from the endoderm develop into four distinct types of cell. Of these cells, copper cells have invaginated microvillar membranes on their apical surface, and they are involved in two distinct functions, i.e., copper absorption and acid secretion. The homeobox gene defective proventriculus (dve) is expressed in the midgut, and two transcripts, type A (similar to 4.9 kb) and type B (similar to 3.5 kb), have been identified. We isolated the deletion allele dve(E181) that completely removes the first exon for type-A (dve-A) transcript. Dve expression pattern in dve-A mutant background indicates that isoform switching is dynamically regulated in a cell-type specific manner. Using RNAi for dve-A, we examined spatial and temporal requirement of the Dve-A activity. Early Dve-A activity is required to repress isoform switching in copper cells, and for establishment of two gut functions. Late Dve-A activity in copper cells, but not in adjacent interstitial cells, is required for acid secretion, while the activity is redundantly required in both cells for the copper absorptive function. Furthermore, ectopic type-B expression in larval copper cells specifically impaired the copper absorptive function. These results provide insight into molecular mechanisms to establish functional specificity. (C) 2011 Elsevier Ireland Ltd. All rights reserved.