Gene transfection and expression in the primary culture of crayfish hemocytes
Gene transfection and expression in the primary culture of crayfish hemocytes
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小龙虾血细胞原代培养中的基因转染及表达
DOI：
10.1016/j.fsi.2011.04.002
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发表时间：
2011-07-01
影响因子：
4.7
通讯作者：
Xu, Limei
中科院分区：
文献类型：
--
作者：
Li, Fang;Ke, Wei;Xu, Limei
缺乏有效的基因传递和表达方法是甲壳动物在细胞水平研究的主要障碍。本文报道了对虾白色斑综合征病毒强启动子表达载体的构建。该载体在原代培养的螯虾血细胞中能高效表达外源基因，电穿孔转染效率在5 ~ 10%之间。本研究为原代螯虾细胞基因功能的体外研究提供了一种方法。(C)2011爱思唯尔有限公司保留所有权利。
Lack of efficient gene delivery and expression methods is the major obstacle for crustacean research in the cellular level. Here, we reported the construction of an expression vector with a strong promoter from shrimp white spot syndrome virus. This vector could efficiently express foreign genes in the primary culture of crayfish hemocytes with the transfection efficiency between 5 and 10% by electroporation. Our findings provide a method for in vitro gene functional study in primary crayfish cells. (C) 2011 Elsevier Ltd. All rights reserved.