The immunoglobulin M heavy chain constant region gene of the channel catfish, Ictalurus punctatus: an unusual mRNA splice pattern produces the membrane form of the molecule.
The immunoglobulin M heavy chain constant region gene of the channel catfish, Ictalurus punctatus: an unusual mRNA splice pattern produces the membrane form of the molecule.
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DOI：
10.1093/nar/18.17.5227
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发表时间：
1990-09
影响因子：
14.9
通讯作者：
Melanie R. Wilson;Ann Marcuz;Frederick van Ginkel;Norman W. Miller;L. Clem;D. Middleton;Gregory W. Warr
中科院分区：
文献类型：
--
作者：
Melanie R. Wilson;Ann Marcuz;Frederick van Ginkel;Norman W. Miller;L. Clem;D. Middleton;Gregory W. Warr
克隆了癍点叉尾（Ictalurus punctatus）免疫球蛋白（IgM）重链恒定区基因。该基因含有四个恒定区结构域编码外显子（CH1至CH4），以分泌形式的免疫球蛋白表达，和两个外显子编码跨膜（TM）结构域，用于淋巴细胞膜受体形式的免疫球蛋白。编码μ链的膜受体形式的信使的3'区的cDNA克隆的序列表明，TM 1外显子直接剪接到CH 3外显子，而不是剪接到CH 4外显子内的位点，如在哺乳动物、鲨鱼和两栖动物中发生的那样。这种不寻常的剪接模式，产生一个膜重链，其特征是小于分泌的重链，可能是共同的所有硬骨鱼。
The immunoglobulin (IgM) heavy chain constant region gene of the channel catfish, Ictalurus punctatus, has been cloned and characterized. The gene contains four constant region domain-encoding exons (CH1 to CH4) expressed in the secreted form of the immunoglobulin, and two exons encoding the transmembrane (TM) domain utilized in the lymphocyte membrane receptor form of the immunoglobulin. The sequence of a cDNA clone encoding the 3' region of the message for the membrane receptor form of the mu chain indicates that the TM1 exon is spliced directly to the CH3 exon, and not into a site within the CH4 exon, as occurs in the mammals, a shark and an amphibian. This unusual pattern of splicing, which produces a membrane heavy chain that is characteristically smaller than the secreted heavy chain, may be common to all teleost fish.