Paenibacillus sputi sp. nov., isolated from the sputum of a patient with pulmonary disease.
Paenibacillus sputi sp. nov., isolated from the sputum of a patient with pulmonary disease.
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DOI：
10.1099/ijs.0.017137-0
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发表时间：
2010-10
影响因子：
2.8
通讯作者：
K. Kim;Keun-chul Lee;Hee-Kyung Yu;S. Ryoo;Youngkil Park;Jung-Sook Lee
中科院分区：
文献类型：
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作者：
K. Kim;Keun-chul Lee;Hee-Kyung Yu;S. Ryoo;Youngkil Park;Jung-Sook Lee
菌株KIT 00200-70066-1（T）分离自肺部疾病患者的痰液。该菌株的细胞是革兰氏可变的、厌氧的、能动的、形成孢子的杆状细胞，并且在30 °C和pH 7下在胰蛋白酶大豆琼脂上形成无色至白色菌落。该菌株的致病性尚不清楚。该菌株含有内消旋二氨基庚二酸作为细胞壁肽聚糖中的诊断性二氨基酸，MK-7作为主要的甲基萘醌，anteiso-C β：l，iso-C β：l和C β：l作为主要脂肪酸，以及二磷脂酰甘油，磷脂酰甘油，磷脂酰乙醇胺和极性脂质谱中的几种未知脂质。基于16 S rRNA基因序列的系统发育分析表明，该菌株属于类芽孢杆菌属（Paenibacillusnanensis MX 2 -3（T），Paenibacillusagaridevorans DSM 1355（T）和Paenibacillusalkaliteratum KSL-134（T）），与它们的同源性最高（分别为95.4、95.2和94.8%），在该属中占有独特的位置。结合系统发育和表型数据支持以下结论：菌株KIT 00200-70066-1（T）代表类芽孢杆菌属的一个新种，拟将其命名为类芽孢杆菌新种;模式菌株为KIT 00200-70066-1（T）（=KCTC 13252（T）=DSM 22699（T））。
Strain KIT 00200-70066-1(T) was isolated from the sputum of a patient with pulmonary disease. Cells of the strain were Gram-variable, facultatively anaerobic, motile, spore-forming rods and formed colourless to white colonies on tryptic soy agar at 30 °C and pH 7. The pathogenicity of the strain is not known. The strain contained meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan, MK-7 as the predominant menaquinone, anteiso-C₁₅:₀, iso-C₁₆:₀ and C₁₆:₀ as the major fatty acids and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and several unknown lipids in the polar lipid profile. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate belongs to the genus Paenibacillus, sharing the highest levels of sequence similarity with Paenibacillus nanensis MX2-3(T), Paenibacillus agaridevorans DSM 1355(T) and Paenibacillus alkaliterrae KSL-134(T) (95.4, 95.2 and 94.8 %, respectively), and that it occupied a distinct position within this genus. Combined phylogenetic and phenotypic data supported the conclusion that strain KIT 00200-70066-1(T) represents a novel species of the genus Paenibacillus, for which the name Paenibacillus sputi sp. nov. is proposed; the type strain is KIT 00200-70066-1(T) (=KCTC 13252(T) =DSM 22699(T)).