ATPase/synthase activity of Paracoccus denitrificans Fo·F1 as related to the respiratory control phenomenon.
ATPase/synthase activity of Paracoccus denitrificans Fo·F1 as related to the respiratory control phenomenon.
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脱氮副球菌Fo·F1的ATP酶/合酶活性与呼吸控制现象有关。
DOI：
10.1016/j.bbabio.2014.04.002
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
A. Vinogradov
中科院分区：
文献类型：
--
作者：
T. V. Zharova;A. Vinogradov
本文研究了副球菌（Paracoccus acidificans）体外膜泡ATP合成、耗氧和膜电位变化的时间过程。ATP的合成开始加入有限量的ADP或无机磷酸盐进行到非常低的残留浓度的限制性底物。积累的ATP并没有降低其合成率启动的ADP。在状态4呼吸测定的残留ADP的量是独立的十倍变化的PIOR的存在下的ATP。PicKm的pH依赖性不能用简单的磷酸解离曲线拟合。部分抑制呼吸导致ATP合成速率下降，而不影响在状态4达到的ATP/ADP。在pH 8.0，水解ATP积累在状态4诱导低浓度的解偶联剂，而完全解偶联的结果在ATP酶的快速失活。在pH 7.0时，未观察到解偶联剂对ATP合酶反应的逆转。数据表明，ATP/ADP × Piratio保持在状态4，与运动产生的驱动力不平衡。对Fo·F1-ATP合酶的动力学控制和单向运作的可能机制进行了讨论。
The time course of ATP synthesis, oxygen consumption, and change in the membrane potential inParacoccus denitrificansinside-out plasma membrane vesicles was traced. ATP synthesis initiated by the addition of a limited amount of either ADP or inorganic phosphate proceeded up to very low residual concentrations of the limiting substrate. Accumulated ATP did not decrease the rate of its synthesis initiated by the addition of ADP. The amount of residual ADP determined at State 4 respiration was independent of ten-fold variation of Pior the presence of ATP. The pH-dependence ofKmfor Picould not be fitted to a simple phosphoric acid dissociation curve. Partial inhibition of respiration resulted in a decrease in the rate of ATP synthesis without affecting the ATP/ADP reached at State 4. At pH 8.0, hydrolysis of ATP accumulated at State 4 was induced by a low concentration of an uncoupler, whereas complete uncoupling results in rapid inactivation of ATPase. At pH 7.0, no reversal of the ATP synthase reaction by the uncoupler was seen. The data show that ATP/ADP × Piratio maintained at State 4 is not in equilibrium with respiratory-generated driving force. Possible mechanisms of kinetic control and unidirectional operation of the Fo·F1-ATP synthase are discussed.
DOI：
10.1016/s0021-9258(18)43822-7
发表时间：
1994-12
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Steven B. VikS;B. Antonio
通讯作者：
Steven B. VikS;B. Antonio
DOI：
10.1016/s0021-9258(18)34936-6
发表时间：
1982-03
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
W E Jacobus;R W Moreadith;K M Vandegaer
通讯作者：
W E Jacobus;R W Moreadith;K M Vandegaer