Regulation of vascular smooth muscle cells on poly(ethylene terephthalate) film by O-carboxymethylchitosan surface immobilization.
Regulation of vascular smooth muscle cells on poly(ethylene terephthalate) film by O-carboxymethylchitosan surface immobilization.
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DOI：
10.1002/jbm.a.31567
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发表时间：
2008-08
期刊：
影响因子：
--
通讯作者：
A. Zhu;F. Zhao;Ning Fang
中科院分区：
文献类型：
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作者：
A. Zhu;F. Zhao;Ning Fang
改变细胞的化学环境是控制细胞行为的公认方法。本研究以聚对苯二甲酸乙二酯（PET）薄膜为典型生物材料，探讨材料表面化学修饰对细胞行为控制的影响。将天然生物高分子壳聚糖及其生物相容性衍生物O-羧甲基壳聚糖（OCMCS）分别通过氩等离子体表面固定在PET上，然后与丙烯酸（AAc）接枝共聚，将PET与壳聚糖（CS）和OCMCS分子共价偶联。平滑肌细胞（SMCs）表现出表面依赖的细胞伸展和细胞骨架组织。培养24 h后，细胞在OCMC修饰的PET表面上比在PET或PAA和壳聚糖固定的PET表面上具有更明显的细长纺锤形、更小的细胞面积和更低的细胞形状指数（CSI）。5天后的细胞培养活力表明，所有改性材料具有良好的细胞增殖。我们的研究结果表明，细胞粘附，形态和生长不仅可以通过改变PET表面的官能团，电荷和润湿性，而且还可以通过从生物材料中引起的特定生物识别来介导。这些发现有力地支持了微环境显著影响细胞行为的概念，突出了环境材料生物化学在基于细胞的组织工程方案中的重要性。
Specifying the chemical environment of cells is a well-established method of controlling cellular behaviors. In this study, poly(ethylene terephthalate) (PET) film was selected as a typical biomaterial to detect the effects of chemical modifications on material surface in controlling cell behaviors. Natural biopolymer chitosan and its biocompatible derivative, O-carboxymethylchitosan (OCMCS) were surface immobilized on PET, respectively, via argon plasma followed by graft copolymerization with acrylic acid (AAc), which was exploited to covalently couple PET with chitosan (CS) and OCMCS molecules. Smooth muscle cells (SMCs) displayed a surface-dependent cell spreading and cytoskeletal organization. The cells spread with a more pronounced elongated spindle shape, smaller cell area, and lower cell shape index (CSI) on OCMCS-modified PET surface than on PET, or the PAA and chitosan-immobilized PET surfaces after 24 h of culture. Cell-culture viability after 5 days showed that all the modified materials possessed good cell proliferation. Our results suggest that cell adhesion, morphology, and growth can be mediated not only by varying the functional groups, electric charge, and wettability of PET surface but also by the specific biological recognition elicited from the biomaterials. These findings strongly support the concept that the microenvironment significantly influences cell behavior, highlighting the importance of environmental material biochemistry in cell-based tissue engineering schemes.