Exogenous ethylene influences flower opening of cut roses (Rosa hybrida) by regulating the genes encoding ethylene biosynthesis enzymes
Exogenous ethylene influences flower opening of cut roses (Rosa hybrida) by regulating the genes encoding ethylene biosynthesis enzymes
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DOI：
10.1360/062004-37
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发表时间：
2005-10-01
期刊：
影响因子：
--
通讯作者：
Gao, JP
中科院分区：
文献类型：
--
作者：
Ma, N;Cai, L;Gao, JP
研究了乙烯对月季切花开放过程的促进作用和抑制作用。首先测定乙烯产生和1-氨基环丙烷-1-羧酸（ACC）合酶和氧化酶活性。乙烯处理后，“Samantha”花瓣中乙烯释放量、ACC合成酶（ACS）和ACC氧化酶（ACO）活性增加，在瓶插前期（第3期）达到峰值，“Kardinal”花瓣中ACS和ACO活性增加幅度更大，在瓶插后期（第4期）达到峰值。克隆了3个Rh-ACS和1个Rh-ACO基因的cDNA片段，分别命名为Rh-ACS 1、Rh-ACS 2、Rh-ACS 3和Rh-ACO 1。北方印迹分析表明，在ACS的3个基因中，乙烯诱导的Rh-ACS 3基因的表达模式与两个品种的ACS活性和乙烯产生量相对应。乙烯诱导‘Kardinal’比‘Samantha’更显著地积累Rh-ACS 3 mRNA。0.2mmol/L的STS作为乙烯作用抑制剂，虽然在“Kardinal”中诱导了Rh-ACS 3的瞬时表达，但总体上抑制了两个品种Rh-ACSs和Rh-ACO的表达。结果表明，'Kardinal'对乙烯的敏感性高于'Samantha'，乙烯诱导的Rh-ACS 3表达变化可能与'Samantha'开花加速和'Kardinal'开花抑制有关。另外的结果表明，三个Rh-ACSs基因与花开放和衰老以及受伤的差异。
The purpose of this paper is to investigate the differential responses of flower opening to ethylene in two cut rose cultivars, 'Samantha', whose opening process is promoted, and 'Kardinal', whose opening process is inhibited by ethylene. Ethylene production and 1-aminocyclopropane-1-carboxylate (ACC) synthase and oxidase activities were determined first. After ethylene treatment, ethylene production, ACC synthase (ACS) and ACC oxidase (ACO) activities in petals increased and peaked at the earlier stage (stage 3) in 'Samantha', and they were much more dramatically enhanced and peaked at the later stage (stage 4) in 'Kardinal' than control during vasing. cDNA fragments of three Rh-ACSs and one Rh- ACO genes were cloned and designated as Rh-ACS1, Rh-ACS2, Rh-ACS3 and Rh-ACO1 respectively. Northern blotting analysis revealed that, among three genes of ACS, ethylene-in- duced expression patterns of Rh-ACS3 gene corresponded to ACS activity and ethylene production in both cultivars. A more dramatic accumulation of Rh-ACS3 mRNA was induced by ethylene in 'Kardinal' than that of 'Samantha'. As an ethylene action inhibitor, STS at concentration of 0.2 mmol/L generally inhibited the expression of Rh-ACSs and Rh-ACO in both cultivars, although it induced the expression of Rh-ACS3 transiently in 'Kardinal'. Our results suggests that 'Kardinal' is more sensitive to ethylene than 'Samantha'; and the changes of Rh-ACS3 expression caused by ethylene might be related to the acceleration of flower opening in 'Samantha' and the inhibition in 'Kardinal'. Additional results indicated that three Rh-ACSs genes were differentially associated with flower opening and senescence as well as wounding.