Identification of a laminin-like substance on the surface of high-malignant murine fibrosarcoma cells.
Identification of a laminin-like substance on the surface of high-malignant murine fibrosarcoma cells.
复制标题
高恶性鼠纤维肉瘤细胞表面层粘连蛋白样物质的鉴定。
作者：
J. McCoy;R. Lloyd;M. Wicha;J. Varani
用免疫过氧化物酶技术对高度恶性和低恶性的小鼠纤维肉瘤细胞进行抗层粘连蛋白抗体染色，并进行电子显微镜观察。对于高度恶性的细胞，沿细胞表面可见特异性染色。用正常兔血清代替兔抗层粘连蛋白，或用可溶性层粘连蛋白对抗层粘连蛋白进行预处理，完全消除了这种染色。低度恶性细胞未见免疫过氧化物酶染色。在另一项研究中，从高度和低恶性细胞中制备了膜组分，并用于免疫兔子。用高度恶性细胞的膜部分免疫的动物产生抗体，通过酶联免疫吸附试验(ELISA)与从EHS肉瘤获得的鼠层粘连蛋白反应。用来自低度恶性细胞的膜部分免疫的动物则没有。这些研究提供了强有力的证据，证明高度恶性的细胞(但不是低恶性细胞)在其细胞表面表达一种与层粘连蛋白发生免疫交叉反应的物质。此外，高度恶性的细胞(但不是低恶性的)会将一种物质分泌到细胞培养液中，这种物质可以与抗层粘连蛋白抗体特异性地免疫沉淀。在还原条件下，经十二烷基硫酸钠/聚丙烯酰胺凝胶电泳法检测，免疫共沉淀物与纯化的层粘连蛋白共迁移。这种与高度恶性细胞表面相关的物质的存在和与低度恶性细胞表面相关物质的缺失可能解释了先前提到的这些细胞在附着IV型胶原的能力上的差异。这种差异也可能是这些细胞转移潜力不同的原因之一。
High- and low-malignant murine fibrosarcoma cells were stained with anti-laminin antibodies using immunoperoxidase techniques and examined by electron microscopy. With the high-malignant cells, specific staining was observed along the cell surface. Use of normal rabbit serum in place of the rabbit anti-laminin or pretreatment of the anti-laminin with soluble laminin completely eliminated this staining. No immunoperoxidase staining was observed with the low-malignant cells. In additional studies, membrane fractions were prepared from the high- and low-malignant cells and used to immunize rabbits. The animals immunized with the membrane fractions from the high-malignant cells produced antibodies that reacted by enzyme-linked immunosorbent assay (ELISA) with murine laminin obtained from the EHS sarcoma. The animals immunized with membrane fractions from the low-malignant cells did not. These studies provide strong evidence that the high-malignant cells (but not the low) express on their cell surface a substance that is immunologically cross-reactive with laminin. In addition, the high-malignant cells (but not the low) secreted a material into the cell culture fluid that could be specifically immunoprecipitated with antilaminin antibodies. The immunoprecipitated material co-migrated with purified laminin when examined by sodium dodecyl sulphate/polyacrylamide gel electrophoresis under reducing conditions. The existence of this substance associated with the surface of the high-malignant cells and its absence from that of the low-malignant cells may explain the previously noted difference between these cells in their ability to attach to type IV collagen. This difference may also contribute to the dissimilarity between these cells in their metastatic potential.
啮齿动物组织中巢蛋白（一种硫酸化基底膜成分）的免疫定位，并与 GP-2（层粘连蛋白）进行比较。
DOI：
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发表时间：
1981
期刊：
The American journal of pathology
影响因子：
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作者：
Bender,BL;Jaffe,R;Carlin,B;Chung,AE
通讯作者：
Chung,AE