The influence of a second ground electrode on hydrogen peroxide production from an atmospheric pressure argon plasma jet and correlation to antibacterial efficacy and mammalian cell cytotoxicity
The influence of a second ground electrode on hydrogen peroxide production from an atmospheric pressure argon plasma jet and correlation to antibacterial efficacy and mammalian cell cytotoxicity
复制标题
DOI：
10.1088/1361-6463/ac43d9
复制
发表时间：
2022-03-24
影响因子：
3.4
通讯作者：
Short, Robert D.
中科院分区：
文献类型：
--
作者：
Ghimire, Bhagirath;Patenall, Bethany L.;Short, Robert D.
本研究探讨在氩电浆射流中加入第二接地电极对去离子水产生过氧化氢的影响。简言之，通过以1升/分（-1）吹扫氩气通过石英管并以23.5kHz施加7 kV（峰-峰）的正弦电压到密封在石英管内的高压不锈钢针电极来点燃等离子体，所述高压不锈钢针电极由一个或两个铜环围绕，所述铜环用作位于高压电极下游的接地电极。H2 O2产生的机制进行了研究，通过处理DIW的电气和光学等离子体特性和化学分析。我们发现，第二接地电极的添加导致在等离子体射流组件的石英管的内壁表面上的电荷的更高的积累，从而导致在放电电流和耗散功率的增加。这进一步导致电子温度的增加，这使通过水蒸气分子的解离重组产生的H2 O2增加一倍以上，同时仍然保持生物组织可耐受的气体温度。双接地电极等离子体射流被证明是非常有效的减少常见的伤口病原体（铜绿假单胞菌和金黄色葡萄球菌）的生长，同时在某些条件下诱导低水平的细胞毒性的HaCaT角质形成细胞的皮肤样细胞在两个渗透性和生物膜状态。在这项研究中提供的信息是有用的，在了解复杂的物理化学过程，影响过氧化氢生产等离子体射流，这是需要优化等离子体源的发展，用于临床应用。
This study investigates how addition of a 2nd ground electrode in an argon plasma jet influences the production of hydrogen peroxide (H2O2) in deionised water (DIW). Briefly, plasma is ignited by purging argon gas through a quartz tube at 1 l min(-1) and applying a sinusoidal voltage of 7 kV (peak-peak) at 23.5 kHz to a high voltage stainless steel needle electrode sealed inside the quartz tube surrounded by one or two copper ring(s) that served as the ground electrode(s) situated downstream of the high voltage electrode. The mechanisms of H2O2 production are investigated through the electrical and optical plasma properties and chemical analysis of the treated DIW. We discover that the addition of a 2nd ground electrode results in higher accumulation of charges on the inner wall surface of the quartz tube of the plasma jet assembly resulting in an increase in the discharge current and dissipated power. This further leads to an increase in the electron temperature that more than doubles the H2O2 production through dissociative recombination of water vapour molecules, whilst still maintaining a biological tissue tolerable gas temperature. The double ground electrode plasma jet is shown to be highly effective at reducing the growth of common wound pathogens (Pseudomonas aeruginosa and Staphylococcus aureus) in both planktonic and biofilm states whilst inducing a low level of cytotoxicity in HaCaT keratinocyte skin-like cells under certain conditions. The information provided in this study is useful in understanding the complex physicochemical processes that influence H2O2 production in plasma jets, which is needed to optimise the development of plasma sources for clinical applications.