E2F4 regulates the cell cycle and DNA replication in the silkworm, Bombyx mori
E2F4 regulates the cell cycle and DNA replication in the silkworm, Bombyx mori
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E2F4 调节家蚕 (Bombyx mori) 的细胞周期和 DNA 复制
DOI：
10.1111/1744-7917.12991
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发表时间：
2022
期刊：
影响因子：
4
通讯作者：
Cheng Lu
中科院分区：
文献类型：
--
作者：
Peng Chen;Ling Wang;Yan‐Bi Long;Guang‐Yan Liang;Xiu Yang;Zhan‐Qi Dong;Xia Jiang;Yan Zhu;Min‐Hui Pan;Cheng Lu
转录因子E2 F家族对细胞周期进程和细胞命运决定至关重要。虽然E2 Fs在哺乳动物中已被广泛研究，但在昆虫中进行的研究很少。本研究对家蚕E2 F4基因的功能进行了初步的研究。我们证明了E2 F蛋白在从低等动物到高等哺乳动物的物种之间是高度保守的。BmE 2F 4基因的过表达导致细胞周期停滞在G1期，而干扰BmE 2F 4 mRNA导致细胞积累在S期。这些结果表明BmE 2F 4在细胞周期调控中是重要的。我们还证明了BmE 2F 4基因参与BmN-SWU 1细胞的DNA复制和丝腺中的DNA合成。此外，我们还发现了一种名为Bm 14 - 3 - 3的蛋白质，它可以与BmE 2F 4相互作用，并使其定位在细胞核中。过表达Bm 14 - 3 - 3 β基因导致细胞周期停滞在G1期，而敲除该基因增加了S期细胞的比例。这些发现为E2 F转录因子的功能提供了重要的见解，并增加了我们对它们参与细胞周期调控的理解。
The E2F family of transcription factors is crucial for cell cycle progression and cell fate decisions. Although E2Fs have been widely studied in mammals, there have been few studies performed in insects. Here, we determined the function of E2F4 in the silkworm, Bombyx mori. We demonstrate that E2F proteins are highly conserved among species from lower animals to higher mammals. Overexpression of the BmE2F4 gene led to cell cycle arrest in the G1 phase, whereas interfering with the BmE2F4 mRNA led to accumulation of cells in the S phase. These results indicate that BmE2F4 is important in cell cycle regulation. We also demonstrate that the BmE2F4 gene is involved in DNA replication of BmN‐SWU1 cells and DNA synthesis in the silk gland. Furthermore, we identified a protein called Bm14‐3‐3ζ that can interact with BmE2F4 and allow it to localize in the nucleus. Overexpression of the Bm14‐3‐3ζ gene led to cell cycle arrest in the G1 phase, while knocking down the gene increased the proportion of cells in S phase. These findings provide important insights into the function of E2F transcription factors and increase our understanding of their involvement in cell cycle regulation.