Genomic and genetic relationships among species of Leymus (Poaceae: Triticeae) inferred from 18S-26S ribosomal genes
Genomic and genetic relationships among species of Leymus (Poaceae: Triticeae) inferred from 18S-26S ribosomal genes
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DOI：
10.2307/2657053
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发表时间：
2001-04-01
影响因子：
3
通讯作者：
Bödvarsdóttir, SK
中科院分区：
文献类型：
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作者：
Anamthawat-Jónsson, K;Bödvarsdóttir, SK
利用荧光原位杂交（FISH）和限制性片段长度多态性（RFLP）技术对3种近缘赖草属植物的18 S-26 S核糖体基因进行了分析。这两种方法都揭示了L. arenarius（8x = 56，北方欧洲）和L.总状花序（4x = 28.中欧亚），而L. mollis（4x = 28，北方美洲/太平洋）是不同的。每个种都有三对同源的主要rDNA位点：L. a1，a2和a3。阿雷纳留斯：我。m2，L. mollis：和L的r1、r2和r3。总状花序。此外，赖草有三个次要位点，a4，a5和a6。沙生乳杆菌和总状乳杆菌的主基因座完全相同，表明沙生乳杆菌可能是通过种间杂交和/或多倍性起源于总状乳杆菌。rDNA-RFLPs进一步揭示了这些物种与赖草属其它物种的亲缘关系。karellini，8x = 56和L. angustus，12 x = 84）和新麦草属（Psathyrostachys）（P. fragilis，huashanica，P. juncea，P.lanana.由20个BamHI、EcoRI和DraI rDNA片段构建的表型图显示，赖草属和新麦草属之间的亲缘关系比属内种间的亲缘关系更近。
The 18S-26S ribosomal genes in three closely related species of Leymus (Poaceae: Triticeae) were examined using fluorescence in situ hybridization (FISH) and restriction fragment length polymorphism (RFLP). Both approaches revealed a close relationship between L. arenarius (8x = 56, northern European) and L. racemosus (4x = 28. central Eurasian), whereas L. mollis (4x = 28, northern American/Pacific) was distinct. Each species had three homologous pairs of major rDNA loci: a1, a2, and a3 for L. arenarius: mi. m2, and m3 for L. mollis: and r1, r2, and r3 for L. racemosus. Leymus arenarius had in addition three minor loci, a4, a5, and a6. The major loci of L arenarius and L, racemosus were identical, indicating that the former species could have originated from the latter; via interspecific hybridization and/or polyploidy. The rDNA-RFLPs further indicated relationships of these species to other species of Leymus (L. karellini, 8x = 56 and L. angustus, 12x = 84) and Psathyrostachys (P. fragilis, huashanica, P. juncea, and P, lanuginosa, which are all diploids). A phenogram constructed from 20 BamHI, EcoRI, and DraI rDNA fragments revealed closer relationship between the two genera, Leymus and Psathyrostachys, than that among species within a genus.