Diversified exploitation of aerolysin nanopore in single-molecule sensing and protein sequencing
Diversified exploitation of aerolysin nanopore in single-molecule sensing and protein sequencing
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DOI：
10.1002/viw.20200006
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发表时间：
2020-12-01
期刊：
影响因子：
8.6
通讯作者：
Long, Yi-Tao
中科院分区：
文献类型：
--
作者：
Lu, Si-Min;Wu, Xue-Yuan;Long, Yi-Tao
纳米孔电化学已经成为一种很有前途的技术，可以在单分子水平上以无标记的方式实现纳米尺度过程的实时研究。为进一步拓展纳米孔单生物分子界面单分子传感的通用性，开发了突变型气溶素纳米孔，并对其传感机理进行了探索。精巧的设计使每个氨基酸沿着气溶素单分子界面进行定点控制，具有化学基团的精度。由于多种化学基团之间的弱相互作用（如静电相互作用、氢键、范德华力）和空间限制效应，突变型气溶素在随机感知过程中对单个分析物表现出极高的灵敏度和选择性。这些精心设计的纳米孔为生物物理、诊断应用提供了更多的机会，甚至成为单分子蛋白质测序的有希望的候选者。
Nanopore electrochemistry has emerged as a promising technology to achieve the real-time investigation of nanoscale processes at the single-molecule level in a label-free manner. To further extend the single-molecule sensing versatility of nanopore single-biomolecule interface, mutant aerolysin nanopore is developed followed by exploring the sensing mechanism. The exquisite design enables the site-directed controlling of every amino acid along the aerolysin single-biomolecule interface with the precision of chemical group. Owing to diversified pore-analyte weak interactions between chemical groups (e.g., electrostatic interactions, hydrogen bond, van der Waals' force) and steric confined effect, the mutant aerolysin displays extraordinarily high sensitivity and selectivity toward individual analytes in the stochastic sensing process. These well-designed nanopores open more opportunities for biophysical, diagnostic applications, and even emerge as promising candidates for single-molecule protein sequencing.