Primary Structure of a γ Subunit of G Protein, γ12, and Its Phosphorylation by Protein Kinase C *
Primary Structure of a γ Subunit of G Protein, γ12, and Its Phosphorylation by Protein Kinase C *
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G 蛋白 γ 亚基 γ12 的一级结构及其被蛋白激酶 C 磷酸化 *
DOI：
--
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发表时间：
1995
影响因子：
4.8
通讯作者：
T. Asano
中科院分区：
文献类型：
--
作者：
R. Morishita;H. Nakayama;T. Isobe;T. Matsuda;Y. Hashimoto;T. Okano;Y. Fukada;K. Mizuno;S. Ohno;O. Kozawa;Kanefusa Kato;T. Asano
We have determined the primary structure of a novel γ subunit (γ12, previously designated γS1) of G protein purified from bovine spleen. The mature γ12 protein composed of 68 amino acids had acetylated serine at the N terminus and geranylgeranylated/carboxylmethylated cysteine at the C terminus. This was consistent with the C-terminal prenylation signal in the amino acid sequence, which was predicted from γ12 cDNA isolated from a bovine spleen cDNA library. Western blots with the specific antibody against γ12 showed that γ12 is present in all tissues examined. Among various γ subunits (γ 1, γ 2, γ 3, γ 7, and γ12), γ12 has a unique property to be phosphorylated by protein kinase C. The phosphorylated amino acid residue was Ser1 (or Ser2). The phosphorylated βγ12 associated with Goα more tightly than the unphosphorylated form. Exposure of Swiss 3T3 and aortic smooth muscle cells to phorbol 12-myristate 13-acetate and NaF induced phosphorylation of γ12. Stimulation of aortic smooth muscle cells with natural vasoactive agents such as angiotensin II and vasopressin also induced phosphorylation of γ12. The extent of phosphorylation of βγ12in vitro was suppressed by a complex formation with Goα, which was relieved by the addition of guanosine 5′-O-(3-thiotriphosphate) or aluminum fluoride. These results strongly suggest that γ12 is phosphorylated by protein kinase C during activation of receptor(s) and G protein(s) in living cells.
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大脑中存在 G 蛋白的两个 γ 亚基。
DOI：
--
发表时间：
1989
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Robishaw,JD;Kalman,VK;Moomaw,CR;Slaughter,CA
通讯作者：
Slaughter,CA
G 蛋白 γ 亚基的选择性组织分布，包括通过 cDNA 克隆鉴定的新形式的 γ 亚基。
DOI：
--
发表时间：
1992
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Cali,JJ;Balcueva,EA;Rybalkin,I;Robishaw,JD
通讯作者：
Robishaw,JD
与视紫红质的特异性相互作用取决于 G 蛋白中的 γ 亚基类型。
DOI：
--
发表时间：
1993
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Kisselev,O;Gautam,N
通讯作者：
Gautam,N
鸡和牛肉豆蔻酰化富含丙氨酸的 C 激酶底物蛋白（蛋白激酶 C 的重要细胞底物）中磷酸化位点的表征。
DOI：
--
发表时间：
1989
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Graff,JM;Stumpo,DJ;Blackshear,PJ
通讯作者：
Blackshear,PJ
G 蛋白 α-γ 亚基相互作用的特异性。
DOI：
10.1074/jbc.270.7.2946
发表时间：
1995
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Rahmatullah,M;Ginnan,R;Robishaw,JD
通讯作者：
Robishaw,JD